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Merck

DNAQF

Sigma-Aldrich

DNA Quantitation Kit, Fluorescence Assay

Quantitation of DNA using bisBenzimide

Sinónimos:

dsDNA quantification

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1 KIT
$233.000

$233.000


Fecha estimada de envío06 de abril de 2025



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1 KIT
$233.000

About This Item

UNSPSC Code:
41105500
NACRES:
NA.84

$233.000


Fecha estimada de envío06 de abril de 2025


Quality Level

usage

sufficient for 750 assays (2 ml each)

technique(s)

nucleic acid detection: suitable

storage temp.

−20°C

General description

The kit has all the reagents necessary for quantitation of DNA using the fluorescent dye bisBenzimide (Hoechst 33258). The kit allows highly sensitive, quick, and accurate determinations of double-stranded DNA from 10ng/ml to 5μg/ml, even in the presence of RNA and proteins.[1][2][3] Fluorometry is a highly sensitive and simple method used for DNA quantitation. The fluorescent dye, bisBenzimide H 33258, which binds primarily to AT sequences in the minor groove of double-stranded DNA (dsDNA), is specific for quantitation of nanogram amounts of DNA (10 ng/ml to 10 mg/ml). When excited at 360 nm, the fluorescence emission at 460 nm of the dye increases significantly in the presence of DNA.

application

DNA Quantitation Kit, Fluorescence Assay has been used to measure the concentration/content of DNA:
  • in neonatal rat ventricular myocytes (NRVMs)[4]
  • in human mesenchymal stem cells (hMSCs)/samples for normalization of the relative alkaline phosphatase (ALP) amount[5]
  • in mouse embryonic fibroblast cell line (NIH3T3 cells)[6]

Features and Benefits

  • Specific for the quantitation of nanogram amounts of DNA
  • Works well with purified preparations as well as with DNA from crude extracts containing RNA and proteins

Solo componentes del kit

Referencia del producto
Descripción

  • Detailed technical bulletin 1 ea

related product

Referencia del producto
Descripción
Precios

Storage Class

10 - Combustible liquids


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The Effect of Pericellular Oxygen Levels on Proteomic Profile and Lipogenesis in 3T3-L1 Differentiated Preadipocytes Cultured on Gas-Permeable Cultureware.
Weiszenstein M
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Claire G Jeong et al.
Journal of biomedical materials research. Part A, 100(8), 2088-2096 (2012-05-23)
As articular cartilage is avascular, and mature chondrocytes do not proliferate, cartilage lesions have a limited capacity for regeneration after severe damage. The treatment of such damage has been challenging due to the limited availability of autologous healthy cartilage and
J M Teare et al.
BioTechniques, 22(6), 1170-1174 (1997-06-01)
Molecular biology is now a routine tool in almost all biological research fields. With the exponential growth in the number of molecular biological techniques, there is a recognizable need for sensitive, accurate and precise quantitation of nucleic acids. We present
Steve Elder et al.
Journal of biomedical materials research. Part A, 106(8), 2251-2260 (2018-03-27)
Given the limited availability of fresh osteochondral allografts and uncertainty regarding performance of decellularized allografts, this study was undertaken as part of an effort to develop an osteochondral xenograft for articular cartilage repair. The purpose was to evaluate a simple
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PloS one, 7(6), e39430-e39430 (2012-06-30)
It has been suggested that cells that are independent of insulin for glucose uptake, when exposed to high glucose or other nutrient concentrations, manifest enhanced mitochondrial substrate oxidation with consequent enhanced potential and generation of reactive oxygen species (ROS); a

Questions

1–4 of 4 Questions  
  1. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  2. I see that one of the componets of Product DNAQF, DNA Quantitation Kit, Fluorescence Assay, is a DNA standard. How is the DNA standard prepared? Can it be purchased separately?

    1 answer
    1. The DNA standard (Product No. D4810) used in the kit is a sheared calf thymus DNA. It is the only one tested with the kit and it is only available in the kit. We do not determine the molecular weight.

      Helpful?

  3. What quality of genomic DNA is required for use in Product DNAQF, DNA Quantitation Kit, Fluorescence Assay?

    1 answer
    1. Genomic DNA purified by phenol chloroform extraction is sufficiently clean for use with this kit.

      Helpful?

  4. How can I ensure accurate initial quantification of the DNA standard by spectrophotometric measurement at A260 when using Product DNAQF, DNA Quantitation Kit, Fluorescence Assay?

    1 answer
    1. The DNA standard (Product No. D4810) should be incubated at 50 °C for 15-30 minutes before determining the concentration spectrophotometrically. This will ensure complete dissolution of the genomic DNA.

      Helpful?

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