Suppression of murine experimental autoimmune encephalomyelitis development by 1,25-dihydroxyvitamin D3 with autophagy modulation.

Suppression of murine experimental autoimmune encephalomyelitis development by 1,25-dihydroxyvitamin D3 with autophagy modulation.

Journal of neuroimmunology (2015-03-17)

Chao Zhen, Xuedan Feng, Zhe Li, Yabo Wang, Bin Li, Lin Li, Moyuan Quan, Gaoning Wang, Li Guo

ABSTRACT

Multiple sclerosis (MS) has been associated with a history of sub-optimal exposure to ultraviolet light, implicating vitamin D3 as a possible protective agent. We evaluated whether 1,25(OH)2D3 attenuates the progression of experimental autoimmune encephalomyelitis (EAE), and explored its potential mechanisms. EAE was induced in C57BL/6 mice via immunization with MOG35-55, and some mice received 1,25(OH)2D3. 1,25(OH)2D3 inhibited EAE progression. Additionally, 1,25(OH)2D3 reduced inflammation, demyelination, and neuron loss in the spinal cord. The protective effect of 1,25(OH)2D3 was associated with significantly elevated expression of Beclin1, increased Bcl-2/Bax ratio, and decreased LC3-II accumulation. Thus, 1,25(OH)2D3 may represent a promising new MS treatment.

MATERIALI

N° Catalogo

Marchio

Descrizione del prodotto

L3771

Sigma-Aldrich

Sodio dodecil solfato, BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)

S0389

Sigma-Aldrich

Saccarosio, for molecular biology, ≥99.5% (GC)

S9378

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Saccarosio, ≥99.5% (GC)

75746

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Sodio dodecil solfato, ≥99.0% (GC), dust-free pellets

S7903

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Saccarosio, ≥99.5% (GC), BioXtra

84097

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Saccarosio, BioUltra, for molecular biology, ≥99.5% (HPLC)

71736

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Sodio dodecil solfato, BioUltra, for molecular biology, 10% in H₂O

1623637

USP

Saccarosio, United States Pharmacopeia (USP) Reference Standard

05030

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Sodio dodecil solfato, BioUltra, for molecular biology, 20% in H₂O

71725

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Sodio dodecil solfato, BioUltra, for molecular biology, ≥99.0% (GC)

D1530

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1α,25-diidrossivitamina D₃, ≥99% (HPLC)

S1888

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Saccarosio, ≥99.5% (GC), BioReagent, suitable for cell culture, suitable for insect cell culture

S8501

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Saccarosio, ≥99.5% (GC)

S5391

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Saccarosio, ≥99.5% (GC), Grade II, suitable for plant cell culture

74255

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Sodio dodecil solfato, dust-free pellets, suitable for electrophoresis, for molecular biology, ≥99.0% (GC)

436143

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Sodio dodecil solfato, ACS reagent, ≥99.0%

71729

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Sodio dodecil solfato, ≥98.0% (GC)

S5390

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Saccarosio, Grade I, ≥99% (GC), suitable for plant cell culture

PHR1001

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Saccarosio, Pharmaceutical Secondary Standard; Certified Reference Material

L4509

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Sodio dodecil solfato, ReagentPlus^®, ≥98.5% (GC)

S3929

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Saccarosio, meets USP testing specifications

71717

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Sodio dodecil solfato, tested according to NF, mixture of sodium alkyl sulfates consisting mainly of sodium dodecyl sulfate

S5016

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Saccarosio, ACS reagent

84100

Millipore

Saccarosio, suitable for microbiology, ACS reagent, ≥99.0%

17936

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1α,25-diidrossivitamina D₃, ≥97.0% (HPLC)

16104

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Saccarosio, puriss., meets analytical specification of Ph. Eur., BP, NF

L6026

Sigma-Aldrich

Sodio dodecil solfato, BioXtra, ≥99.0% (GC)

H-090

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1α,25-Dihydroxyvitamin D2 solution, 5 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant^®

L5750

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Sodio dodecil solfato, 92.5-100.5% based on total alkyl sulfate content basis

71726

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Sodio dodecil solfato, suitable for ion pair chromatography, LiChropur^™, ≥99.0%