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SRP3014

Sigma-Aldrich

BDNF human

Carrier free, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), suitable for cell culture

Sinonimo/i:

Brain Derived Neurotrophic Factor

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About This Item

Codice UNSPSC:
12352202
NACRES:
NA.32

Origine biologica

human

Livello qualitativo

Ricombinante

expressed in E. coli

Saggio

≥95% (SDS-PAGE)

Forma fisica

lyophilized

PM

27.0 kDa

Confezionamento

pkg of 10 μg

tecniche

cell culture | mammalian: suitable

Impurezze

≤1.00 EU/μg endotoxin, tested

N° accesso UniProt

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... BDNF(627)

Descrizione generale

The gene BDNF (brain derived neurotrophic factor) is mapped to human chromosome 11p14.1. BDNF is a member of the neurotrophin family of growth factors. The gene encodes a precursor protein, proBDNF. Mature BDNF (mBDNF) is synthesized by post-translational cleavage of proBDNF. Both proBDNF and mBDNF play crucial roles in cellular signaling. BDNF is expressed as the C-terminal portion of a 247 amino acid polypeptide precursor, which also contains a signal sequence of 18 amino acid residue and a propeptide of 110 amino acid residues. Recombinant human BDNF is a 27.0kDa homodimer of two 119 amino acid subunits linked by strong noncovalent interactions.

Azioni biochim/fisiol

Like other members of this family, BDNF (brain derived neurotrophic factor) supports neuron proliferation and survival. BDNF can bind to a low affinity cell surface receptor called LNGFR (low-affinity nerve growth factor receptor), which also binds other neurotrophins such as NGF (nerve growth factor), NT-3 (neurotrophin-3) and NT-4. However, BDNF mediates its neurotrophic properties by signaling through a high affinity cell surface receptor called gp145/trkB (tropomyosin-related kinase B). BDNF also plays an important role in vascular function and participates in angiogenesis. It is involved in the pathogenesis of Alzheimer′s disease. ProBDNF interacts with p75 neurotrophin receptor, leading to long-term depression in the hippocampus.

Sequenza

MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR

Stato fisico

Lyophilized with no additives.

Ricostituzione

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

Risultati analitici

The biological activity is determined by its ability to stimulate the proliferation of human neuroblastoma cells, SH-SY5Y.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Function and evolution in the NGF family and its receptors.
Journal of Neuroscience Research, 32, 461-470 (1992)
Nia M Harris et al.
Pharmacology, biochemistry, and behavior, 150-151, 48-56 (2016-10-26)
Low levels of brain-derived neurotrophic factor (BDNF) are linked to delayed neurological recovery, depression, and cognitive impairment following stroke. Supplementation with BDNF reverses these effects. Unfortunately, systemically administered BDNF in its native form has minimal therapeutic value due to its
Cherian K Kandathil et al.
Hearing research, 342, 134-143 (2016-10-25)
Many previous studies have shown significant neurotrophic effects of intracochlear delivery of BDNF in preventing degeneration of cochlear spiral ganglion (SG) neurons after deafness in rodents and our laboratory has shown similar results in developing cats deafened prior to hearing
Elisabetta Palazzo et al.
International journal of molecular sciences, 20(14) (2019-07-25)
Well-regulated epidermal homeostasis depends on the function of different classes of factors, such as transcription regulators and receptors. Alterations in this homeostatic balance may lead to the development of cutaneous squamous tumorigenesis. The homeobox transcription factor DLX3 is determinant for
Jürgen Tuvikene et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 36(4), 1290-1305 (2016-01-29)
Brain-derived neurotrophic factor (BDNF), a member of the neurotrophin family, regulates both survival and differentiation of several neuronal populations in the nervous system during development, as well as synaptic plasticity in the adult brain. BDNF exerts its biological functions through

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