Our lab has not conducted testing on the inhibition of P8465 on PreScission Protease. However, considering that the protein structure of PreScission Protease contains a cysteine nucleophile in its active site and the protein folding resembles that of chymotrypsin-like serine protease, it is likely that the inhibition is caused by ABESF and/or E-64, both of which are included in the cocktail of P8465.
P8465
Protease Inhibitor Cocktail
lyophilized powder, for the inhibition of serine, cysteine, aspartic, metalloproteases and aminopeptidases, for use with bacterial cell extracts, lyophilized powder
Sinonimo/i:
Protease inhibitor
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5 ML
CHF 123.00
25 ML
CHF 507.00
CHF 123.00
Spedizione prevista il23 maggio 2025
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Cambia visualizzazione
5 ML
CHF 123.00
25 ML
CHF 507.00
About This Item
Codice UNSPSC:
12352200
NACRES:
NA.77
CHF 123.00
Spedizione prevista il23 maggio 2025
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Nome del prodotto
Polvere di cocktail di inibitori delle proteasi, for use with bacterial cell extracts, lyophilized powder
Livello qualitativo
Stato
lyophilized powder
Solubilità
water: soluble
Temperatura di conservazione
−20°C
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Descrizione generale
The Protease Inhibitor Cocktail is a lyophilized powder for use in the inhibition of proteases present in bacterial cell extracts.
The product contains individual components that target serine, cysteine, aspartic, and metalloproteases, as well as aminopeptidases.
The product contains individual components that target serine, cysteine, aspartic, and metalloproteases, as well as aminopeptidases.
Applicazioni
The protease inhibitor cocktail improves the yields of intact proteins by adding inhibitors to enzymes that modify proteins present in cell extracts. This product has been optimized and tested for bacterial cell use, with broad specificity against serine, cysteine, and aspartic proteases, metalloproteases, and aminopeptidases.
Azioni biochim/fisiol
This mixture contains individual components, including AEBSF at 23 mM, EDTA at 100 mM, Bestatin at 2 mM, Pepstatin A at 0.3 mM, and E-64 at 0.3 mM. AEBSF acts to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. Pepstatin A inhibits acid proteases. EDTA is an inhibitor of metalloproteases.
Caratteristiche e vantaggi
Broad specificity against serine, cysteine, aspartic, and metalloproteases, as well as aminopeptidases.
Contains individual components, including AEBSF, EDTA, Bestatin, Pepstatin A, and E-64, each targeting specific types of proteases.
Lyophilized powder is stable for at least 2 years when stored unopened at -20°C.
Supplied with a vial of DMSO for preparation of a cocktail solution.
One mL of the cocktail solution is recommended for the inhibition of protease activity found in 20 mL of cell lysate from 4g of E. coli cells.
Contains individual components, including AEBSF, EDTA, Bestatin, Pepstatin A, and E-64, each targeting specific types of proteases.
Lyophilized powder is stable for at least 2 years when stored unopened at -20°C.
Supplied with a vial of DMSO for preparation of a cocktail solution.
One mL of the cocktail solution is recommended for the inhibition of protease activity found in 20 mL of cell lysate from 4g of E. coli cells.
Avvertenza
The lyophilized powder is stable for at least 2 years when stored unopened at -20°C. It is also supplied with a vial of DMSO. A prepared solution in DMSO and water will remain clear and colorless for approximately 24 hours at 4°C, before the inhibitors will precipitate out.
Nota sulla preparazione
A cocktail solution may be prepared by adding 1 mL of DMSO and 4 mL of deionized water to the 5 mL size or 5 mL of DMSO and 20 mL of deionized water to the 25 mL size. One mL of the cocktail solution is recommended for the inhibition of the protease activity found in 20 mL of cell lysate from 4g of E. coli cells.
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Avvertenze
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Indicazioni di pericolo
Classi di pericolo
Acute Tox. 4 Inhalation - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A - STOT RE 2 Inhalation
Organi bersaglio
Respiratory Tract
Codice della classe di stoccaggio
8A - Combustible corrosive hazardous materials
Classe di pericolosità dell'acqua (WGK)
WGK 3
Dispositivi di protezione individuale
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
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Contenuto correlato
Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
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Does the protease inhibitor P8465 inhibit PreScission Protease? We utilized P8465 inhibitors in the purification of a GST-tagged protein fraction, and observed low activity of the PreScission protease (GE27-0843-01) on the dialyzed (using 10 kDa MWCO) GST-tagged protein fraction. Can you provide insight into the inhibition of the PreScission protease by this protease inhibitor cocktail?
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