S8820
Protease Inhibitor Cocktail
SIGMA FAST, tablet, for the inhibition of serine, cysteine, aspartic and metalloproteases, For General Use
Sinonimo/i:
Protease Inhibitor Tablets, protease inhibitor
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About This Item
Codice UNSPSC:
12352200
NACRES:
NA.77
Stato:
tablet
Solubilità:
water: 1 tablet/100mL, clear to hazy, colorless
Temperatura di conservazione:
2-8°C
Prodotti consigliati
Nome del prodotto
SIGMAFAST™ Protease Inhibitor Tablets, For General Use
Origine biologica
bovine lung
bovine milk
Livello qualitativo
Stato
tablet
Solubilità
water: 1 tablet/100mL, clear to hazy, colorless
Temperatura di conservazione
2-8°C
Descrizione generale
SIGMAFAST™ Protease Inhibitor Tablets are a water-soluble tablet formulation containing a broad-spectrum cocktail of protease inhibitors, including AEBSF, E-64, bestatin, leupeptin, aprotinin, and EDTA. One tablet can make 100 mL of cocktail and can inhibit proteases present in up to 20 g of cell extract.
The use of SIGMAFAST Protease Inhibitor Tablets is not advised with HIS-Select® products or other immobilized metal affinity chromatography (IMAC) products. These tablets contain EDTA, which is a metal chelator and can potentially remove metal ions from these affinity products.
Specificità
Inhibits serine, cysteine, aspartic, and metalloproteases.
Applicazioni
SIGMAFAST Protease Inhibitor Tablets has been used:
- to wash raw semen for membrane protein analysis
- in a solution to incubate CD4+ T cells to obtain total cell lysates for western blot analyses
- as a component of NP-40 buffer to homogenize the spinal cord tissue centered at the injury epicenter to conduct western blot analysis
- as a component in radio-immunoprecipitation assay (RIPA) buffer to homogenize the spinal cord tissue centered at the injury center to detect neuregulin-1 beta 1 (Nrg-1ß1) in spinal cord tissue samples with enzyme-linked immunosorbent assay (ELISA)
These tablets have been optimized and tested for general use. They have been used in various applications, including collecting tracheae in combination with ice-cold radioimmunoprecipitation assay buffer to determine the tracheal basal cell fate, and homogenizing rat lung tissues for western blot analysis.
Caratteristiche e vantaggi
- Broad-spectrum protease inhibition: inhibits serine, cysteine, aspartic, and metalloproteases, making it a versatile solution for protease inhibition in various applications.
- Water-soluble tablet formulation: easy to use and prepare, with one tablet making 100 mL of cocktail.
- Contains a cocktail of protease inhibitors: provides a comprehensive and effective solution for protease inhibition.
- Tested and optimized for general use: proven performance and reliability for a wide range of applications.
Componenti
AEBSF, 2 mM
Aprotinin, 0.3 μM
Bestatin, 130 μM
EDTA, 1 mM
E-64, 14 μM
Leupeptin, 1 μM
Aprotinin, 0.3 μM
Bestatin, 130 μM
EDTA, 1 mM
E-64, 14 μM
Leupeptin, 1 μM
Quantità
One tablet makes 100 mL of cocktail. One mL is recommended for the inhibition of proteases equivalent to 1 mg of USP pancreatin. One tablet is recommended for the inhibition of proteases present in a maximum of 20 g of cell extract.
Stato fisico
Tablet that is water soluble.
Altre note
For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
Note legali
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Avvertenze
Danger
Indicazioni di pericolo
Classi di pericolo
Eye Dam. 1 - Skin Corr. 1A - STOT SE 3
Organi bersaglio
Respiratory system
Codice della classe di stoccaggio
8A - Combustible corrosive hazardous materials
Classe di pericolosità dell'acqua (WGK)
WGK 3
Dispositivi di protezione individuale
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
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Articoli
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.
Contenuto correlato
Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
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