P6611

HIS-Select^® Nickel Affinity Gel

(1:1 suspension in a 20% ethanol solution)

• Sinonimo/i: Ni-NTA resin, nickel charged agarose
• Codice UNSPSC: 12352200
• NACRES: NA.56

Proprietà

• Coniugato: magnetic beads
• Livello qualitativo: 200
• Forma fisica: (1:1 suspension in a 20% ethanol solution)
• Caratteristiche: hydrophilic
• Confezionamento: pkg of 1 mL; pkg of 100 mL; pkg of 25 mL; pkg of 5 mL; pkg of 500 mL
• Concentrazione: 1.5-2.4 mL/mL (suspension in packed gel)
• tecniche: protein purification: suitable
• Colore: faint blue to very dark blue
• Matrice: 6% Beaded Agarose
• Capacità: >15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)
• Temp. transizione: flash point 32 °C (closed cup)
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

HIS-Select^® Nickel Affinity Gel is an immobilized metal ion affinity chromatography (IMAC) product, used for the purification of His-tagged proteins. While the unique, non-charged, hydrophilic linkage of the proprietary quadridentate NTA chelate group to the beaded agarose charged with nickel ensures high selectivity for small to medium scale His-tag protein purification, it also results in reduced non-specific binding of other proteins. HIS-Select Nickel Affinity Gel is selective for recombinant proteins with His-tags and exhibits low non-specific binding of other proteins. The selectivity can be modulated with the inclusion of imidazole during chromatography.

HIS-Select^® Nickel Affinity Gel is durable and can capture the recombinant proteins with histidine tags at a high flow rate. Recombinant proteins with histidine tags are bound using either native or denaturing conditions.

Applicazioni

HIS-Select^® Nickel Affinity Gel has been used in the purification of recombinant proteins like EF-hand calcium-binding protein (S100A14), LIM homeobox transcription factor 1 alpha protein, sigma-1 receptor as well as harpin, stable protein 1 (SP1), and BCR-ABL fusion protein.

Caratteristiche e vantaggi

• High selectivity for higher purity.
• Unique non-charged hydrophilic linkage reduces non-specific binding.
• Binding capacity for histidine-tagged protein is greater than 15 mg/mL.
• Binding under denaturing or non-denaturing conditions.
• One-step purification.
• Minimizes unwanted ionic interactions.
• Minimal nickel leaching.
• Bead size: 45-165 μm.

Linkage

It is also available with the EZview™ technology (Product Code E3528).

Stato fisico

1:1 suspension in a 20% ethanol solution

Stoccaggio e stabilità

HIS-Select Nickel Affinity Gel is stable for at least one year when stored properly. The HIS-Select Nickel Affinity Gel should be cleaned after each use and an antimicrobial agent such as 20% ethanol should be added to the storage buffer.

Note legali

HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Pittogrammi: GHS02
• Avvertenze: Warning
• Indicazioni di pericolo: H226
• Consigli di prudenza: P210 - P233 - P240 - P241 - P242 - P243
• Classi di pericolo: Flam. Liq. 3
• Codice della classe di stoccaggio: 3 - Flammable liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): 89.6 °F - closed cup
• Punto d’infiammabilità (°C): 32 °C - closed cup