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MAK113

Sigma-Aldrich

ATPase/GTPase Activity Assay Kit

sufficient for 200 colorimetric tests

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About This Item

Codice UNSPSC:
12161503
NACRES:
NA.84

impiego

sufficient for 200 colorimetric tests

Metodo di rivelazione

colorimetric

Malattie correlate

cancer

Temperatura di conservazione

2-8°C

Descrizione generale

ATPases and GTPases catalyze the decomposition of ATP or GTP into ADP or GDP and free phosphate. These enzymes play key roles in transport, signal transduction, protein biosynthesis, and cell differentiation.

Caratteristiche e vantaggi

Compatible with high-throughput handling systems.

Compatibilità

Suitable for the detection of ATPase and GTPase activity and for the screening of ATPase and GTPase inhibitors.

Principio

The ATPase/GTPase Activity Assay kit provides a simple and direct procedure for measuring ATPase/GTPase activity in a microplate format. This kit uses a single reagent formulation to accurately determine enzyme activity in 30 minutes at room temperature. The malachite green reagent forms a stable dark green color with free phosphate liberated by the enzymes resulting in a colorimetric product, measured at 620 nm (600-660 nm), proportional to the enzyme activity present One unit of activity is the amount of enzyme that catalyzes the production of 1 μmole of free phosphate per minute under the assay conditions.

Pittogrammi

Corrosion

Avvertenze

Warning

Indicazioni di pericolo

Consigli di prudenza

Classi di pericolo

Met. Corr. 1

Codice della classe di stoccaggio

8B - Non-combustible corrosive hazardous materials

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Shuhui Wang et al.
Protein & cell, 11(2), 124-137 (2019-11-24)
Type VII secretion systems (T7SSs) are found in many disease related bacteria including Mycobacterium tuberculosis (Mtb). ESX-1 [early secreted antigen 6 kilodaltons (ESAT-6) system 1] is one of the five subtypes (ESX-1~5) of T7SSs in Mtb, where it delivers virulence
Meng-Yan Sun et al.
Oncology reports, 39(6), 2673-2680 (2018-04-06)
Regulator of G‑protein signaling 1 (RGS1) has been found to be a critical factor in melanoma and other malignancies. However, the mechanism involved in the RGS1‑mediated promotion of melanoma progression is not clear. We based our study on samples collected from
Shannon E Hill et al.
PloS one, 12(7), e0180241-e0180241 (2017-07-26)
Dihydroneopterin triphosphate pyrophosphatase (DHNTPase), a member of the Mg2+ dependent Nudix hydrolase superfamily, is the recently-discovered enzyme that functions in the second step of the pterin branch of the folate biosynthetic pathway in E. coli. DHNTPase is of interest because
Emilie Mahieu et al.
Biophysical journal, 119(2), 375-388 (2020-07-09)
The proteasome is a key player of regulated protein degradation in all kingdoms of life. Although recent atomic structures have provided snapshots on a number of conformations, data on substrate states and populations during the active degradation process in solution
Ru Li et al.
Phytopathology, 109(8), 1417-1424 (2019-03-13)
The vacuolar H+-ATPases (V-ATPases) are conserved ATP-dependent proton pumps that acidify intracellular compartments in eukaryotic cells. The role of Cpvma1, a V-ATPase catalytic subunit A of Cryphonectria parasitica, was investigated by generating cpvma1-overexpressing and cpvma1-silenced strains. The mutant strains were

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