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H8162

Millipore

HIS-Select® Cobalt Affinity Gel

(1:1 suspension in a 30% ethanol solution)

Sinonimo/i:

cobalt affinity gel, cobalt charged agarose

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About This Item

Codice UNSPSC:
41106500
NACRES:
NA.56

Stato

(1:1 suspension in a 30% ethanol solution)

Durata

1 yr (when stored properly.)

tecniche

protein purification: suitable

Matrice

6% Beaded Agarose

Capacità

>15 mg/mL, agarose binding capacity (protein)(with an approx. 30 kDa protein)

Temperatura di conservazione

2-8°C

Categorie correlate

Descrizione generale

The HIS-Select Cobalt Affinity Gel is a quadridentate chelate on beaded agarose that is charged with cobalt. The gel is designed to specifically bind histidine-containing proteins / His-tag proteins. It is selective for recombinant proteins with histidine tags and exhibits low non-specific binding of other proteins.
HIS-Select Cobalt Affinity Gel is an immobilized metal-ion affinity chromatography (IMAC) product. The matrix for this affinity gel is 6% beaded agarose. The selectivity can be modulated with the inclusion of imidazole during chromatography. HIS-Select Cobalt Affinity Gel is durable and can capture recombinant proteins with histidine tags at a high flow rate. Recombinant proteins with His tags are bound using native, denaturing, or mild reducing conditions. The capacity of this affinity gel is typically >15 mg/mL of packed gel, determined with a His-tagged protein of molecular mass ~30 kDa.

Applicazioni

HIS-Select Cobalt Affinity Gel allows for high purity, low non-specific binding, and high binding capacity of protein. HIS-Select Cobalt Affinity Gel also works well for purification of histidine-tagged proteins / His-tag proteins in native, denaturing, or mild reducing conditions. HIS-Select® Cobalt Affinity Gel has been used to purify His-tagged proteins.

Stato fisico

1:1 suspension in a 30% ethanol solution

Note legali

HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

Avvertenze

Danger

Classi di pericolo

Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Irrit. 2 - Flam. Liq. 3 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1

Classe di pericolosità dell'acqua (WGK)

WGK 3

Dispositivi di protezione individuale

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Certificati d'analisi (COA)

Lot/Batch Number
0000428685
0000428686
0000428685
0000428686
0000387154

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Millipore

70691

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CelLytic™ B Cell Lysis Reagent For bacterial cell lysis, standard strength

Sigma-Aldrich

B7435

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Imidazolo ≥99% (titration)

Sigma-Aldrich

I5513

Imidazolo

Katie L Whalen et al.
Journal of chemical information and modeling, 53(9), 2349-2359 (2013-10-12)
Glutamate racemase (GR) is a cofactor independent amino acid racemase that has recently garnered increasing attention as an antimicrobial drug target. There are numerous high resolution crystal structures of GR, yet these are invariably bound to either D-glutamate or very
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Scientific reports, 6, 31933-31933 (2016-08-24)
Cation diffusion facilitators (CDF) are highly conserved, metal ion efflux transporters that maintain divalent transition metal cation homeostasis. Most CDF proteins contain two domains, the cation transporting transmembrane domain and the regulatory cytoplasmic C-terminal domain (CTD). MamM is a magnetosome-associated
Kurt C Curtis et al.
Parasitology research, 120(2), 535-545 (2021-01-09)
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Michael Riis Hansen et al.
Biomolecular NMR assignments, 8(1), 103-108 (2013-01-15)
The type I phosphoribosyltransferase OMP synthase (EC 2.4.2.10) is involved in de novo synthesis of pyrimidine nucleotides forming the UMP precursor orotidine 5'-monophosphate (OMP). The homodimeric enzyme has a Rossman α/β core topped by a base-enclosing "hood" domain and a
Retromer associates with the cytoplasmic amino-terminus of polycystin-2
Tilley F, et al.
Journal of Cell Science, 211342-211342 (2018)
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