P3375
Papain from papaya latex
crude powder, 1.5-10 units/mg solid
Sinonimo/i:
Papainase
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25 G
CHF 92.50
100 G
CHF 293.00
1 KG
CHF 953.00
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Cambia visualizzazione
25 G
CHF 92.50
100 G
CHF 293.00
1 KG
CHF 953.00
About This Item
Prodotti consigliati
Stato
crude powder
Attività specifica
1.5-10 units/mg solid
PM
23.4 kDa
Temperatura di conservazione
2-8°C
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Categorie correlate
Applicazioni
Papain from papaya latex has been used:
- in the enzyme cocktail to evaluate the efficacy of B complex vitamers liberation from nutritional yeast powder[1]
- in the enzyme mixture for the digestion of bovine milk samples for the analysis of three vitamin B1 molecules[2]
- as a biocatalyst for the hydrolysis of (R,S)-naproxen 2,2,2-trifluoroethyl ester in water-saturated solvents[3]
Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies.[4] It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules. Product P3375, papain crude powder, has been used to digest fresh cartilage of dogsfish in order to isolate chondroitin sulfate.
Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.
Azioni biochim/fisiol
A cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.
Papain papaya latex has antifungal activity against C. albicans. It is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
. The pH optimum is 6.0-7.0
. Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.
. The pH optimum is 6.0-7.0
. Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.
Qualità
Not standardized with lactose or other adulterants.
Definizione di unità
One unit will hydrolyze one micromole of N-alpha-benzoyl-l-arginine ethyl ester (BAEE) per minute at pH 6.2 at 25 deg C.
Altre note
View more information on papain at www.sigma-aldrich.com/enzymeexplorer.
Inibitore
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Descrizione
Determinazione del prezzo
Substrato
N° Catalogo
Descrizione
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Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organi bersaglio
Respiratory system
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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I clienti hanno visto anche
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Enzyme and Microbial Technology, 36(1), 127-132 (2005)
Comparison of different extraction methods for simultaneous determination of B complex vitamins in nutritional yeast using LC/MS-TOF and stable isotope dilution assay
Halvin K, et al.
Analytical and Bioanalytical Chemistry, 405(4), 1213-1222 (2013)
Jocelyn L Y Tang et al.
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Determination of the native forms of vitamin B1 in bovine milk using a fast and simplified UHPLC method
Schmidt A, et al.
Food Chemistry, 229, 452-457 (2017)
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One bottleneck in tissue regeneration with hydrogel scaffolds is the limited understanding of the crucial factors for controlling hydrogel's physical microenvironments to regulate cell fate. Here, the effects of permeability and living space of hydrogels on encapsulated cells' behavior were
Articoli
Uncover properties and applications of the cysteine protease papain and find inhibitors, substrates, and other papain products.
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