L0382

Lipase from porcine pancreas

Type VI-S, ≥20,000 units/mg protein, lyophilized powder

• Sinonimo/i: PPL, Triacylglycerol acylhydrolase, Triacylglycerol lipase
• Numero CAS: 9001-62-1
• Classificazione EC (Enzyme Commission): 3.1.1.3 (BRENDA, IUBMB)
• Numero CE: 232-619-9
• Numero MDL: MFCD00131509
• Codice UNSPSC: 12352204
• NACRES: NA.54

Proprietà

• Tipo: Type VI-S
• Livello qualitativo: 300
• Stato: lyophilized powder
• Attività specifica: ≥20,000 units/mg protein
• Composizione: Protein, 40-70%
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;; QWZUIMCIEOCSJF-CHHCPSLASA-N

Descrizione

Descrizione generale

Lipase from porcine pancreas (PPL) or porcine pancreatic lipase is a small globular protein with N-terminal α/β type fold. It comprises catalytic triad Ser, Asp, and His residues and a C-terminal β-sandwich domain.[1] PPL belongs to a subclass of the carboxylesterases family.[2]

Applicazioni

Lipase from porcine pancreas has been used:

• in lipase inhibition assay with plant extracts[3]
• in in vitro digestion gelatinized starch/fiber mixtures[4]
• with simulated intestinal fluid to mimic the digestive tract condition of by L. fermentum PC1 in fermented oats[5]

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Azioni biochim/fisiol

Lipase from porcine pancreas (PPL) catalyzes the hydrolysis of fats (lipids). It plays an essential role in the digestion, transport, and processing of dietary lipids (e.g. triglycerides, fats, oils) in most living organisms.[2] PPL is cost-effective and is useful in biotransformation reactions.[1] It displays high selectivity, and catalytic activity.[1] PPL is also used in the detergent formulation for removing oil stains from clothes.[1]

Lipases catalyze the hydrolysis of esters in aqueous solutions and the synthesis of esters in non-aqueous solutions. They also produce hydroxyl and carboxylic groups through the hydrolysis of ester linkages in poly(ethylene terephthalate).

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Definizione di unità

One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.7 at 37 °C using olive oil.

Risultati analitici

Protein determined by biuret.

Informazioni sulla sicurezza

• Pittogrammi: GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H334
• Consigli di prudenza: P261 - P284 - P501
• Classi di pericolo: Resp. Sens. 1
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)