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I0516

Sigma-Aldrich

Insulina

10 mg/mL insulin in 25  mM HEPES, pH 8.2, BioReagent, sterile-filtered, suitable for cell culture

Sinonimo/i:

Bovine insulin, Cell culture grade insulin

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5 ML
CHF 147.00

CHF 147.00

Spedizione prevista il02 aprile 2025

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5 ML
CHF 147.00

About This Item

Numero CAS:
11070-73-8
Numero MDL:
MFCD00131380
Codice UNSPSC:
12352209
NACRES:
NA.77
Stato:
solution

CHF 147.00

Spedizione prevista il02 aprile 2025

Richiedi un ordine bulk

Sterilità

sterile-filtered

Livello qualitativo

400

Nome Commerciale

BioReagent

Stato

solution

Concentrazione

10 mg/mL insulin in 25  mM HEPES, pH 8.2

tecniche

cell culture | mammalian: suitable
single cell analysis: suitable

Impurezze

endotoxin, tested

pH

7.9-8.5

N° accesso UniProt

P01317

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

Stringa SMILE

SC[C@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CN)[C@H](CC)C)C(C)C)CCC(=O)O)CCC(=O)N)CS)C(=O)N[C@@H](C)C(=O)N[C@@H](CO)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CS)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc4ccc(cc4)O)C(=O)N[

InChI

1S/2C97H151N25O34S4/c2*1-12-46(10)77(120-72(131)35-98)96(154)122-76(45(8)9)94(152)107-55(24-28-74(134)135)79(137)104-53(22-26-69(100)128)82(140)116-66(40-159)93(151)118-64(38-157)90(148)103-47(11)78(136)114-63(37-124)89(147)121-75(44(6)7)95(153)119-67(41-160)92(150)115-62(36-123)88(146)109-57(30-43(4)5)84(142)110-58(31-48-13-17-50(125)18-14-48)85(143)105-52(21-25-68(99)127)80(138)108-56(29-42(2)3)83(141)106-54(23-27-73(132)133)81(139)112-60(33-70(101)129)87(145)111-59(32-49-15-19-51(126)20-16-49)86(144)117-65(39-158)91(149)113-61(97(155)156)34-71(102)130/h2*13-20,42-47,52-67,75-77,123-126,157-160H,12,21-41,98H2,1-11H3,(H2,99,127)(H2,100,128)(H2,101,129)(H2,102,130)(H,103,148)(H,104,137)(H,105,143)(H,106,141)(H,107,152)(H,108,138)(H,109,146)(H,110,142)(H,111,145)(H,112,139)(H,113,149)(H,114,136)(H,115,150)(H,116,140)(H,117,144)(H,118,151)(H,119,153)(H,120,131)(H,121,147)(H,122,154)(H,132,133)(H,134,135)(H,155,156)/t2*46-,47-,52-,53-,54-,55-,56-,57-,58-,59-,60-,61-,62-,63-,64-,65-,66-,67-,75-,76-,77-/m00/s1
XYPANFGXFLOTDK-CJLNJPJHSA-N

Informazioni sul gene

bovine ... INS(280829)
cow ... INS(280829)

Categorie correlate

Applicazioni

La soluzione di insulina da pancreas bovino è stata usata per studiare:
  • l'effetto di insulina esogena sulle concentrazioni plasmatiche di glucosio, insulina, fattore di crescita insulino-simile I (IGF-I) e progesterone (P4) in giovenche in età puberale[1]
  • la responsività all'insulina della placenta nel corso di complicanze gravidiche utilizzando trofoblasti umani primari ed espianti di villi placentari[2]
  • l'effetto della trasduzione del segnale del recettore insulinico sulla densità delle spine dendritiche di neuroni ippocampali neonatali murini in vitro[3]

Azioni biochim/fisiol

L'insulina è uno dei principali ormoni che regolano il glucosio nel sangue e la cui insufficienza causa l'iperglicemia caratteristica del diabete di tipo 2.[4]
Ormone polipeptidico a doppia catena prodotto dalle β-cellule delle isole pancreatiche. Il suo peso molecolare è circa 5800 Da. Le catene α e β sono unite da due legami disolfuro. La catena α contiene un legame disolfuro interno. L′insulina regola la captazione, l′utilizzo e lo stoccaggio di glucosio, aminoacidi e acidi grassi a livello cellulare e inibisce la disgregazione di glicogeno, proteine e grassi.

Stato fisico

stabile a 2-8 °C

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves

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Certificati d'analisi (COA)

Lot/Batch Number
0000263011
0000263011
SLCN8817
SLCJ7615
SLCJ8080

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Insulina

Wafa B'chir et al.
Endocrinology, 159(5), 2153-2164 (2018-04-11)
Given the increasing prevalence of obesity and the metabolic syndrome, identification of intrinsic molecular programs responsible for ensuring fuel homeostasis and preventing metabolic disease is needed. We investigated whether the orphan nuclear receptor estrogen-related receptor α (ERRα), a major regulator
M M Vick et al.
Journal of animal science, 85(5), 1144-1155 (2007-02-01)
Recent studies associate obesity and insulin resistance in horses with development of abnormal reproductive function and debilitating laminitis. The factors contributing to insulin resistance in obese horses are unknown. However, human studies provide evidence that elevated inflammatory cytokines such as
Ana Mato-Berciano et al.
Oncotarget, 8(14), 22700-22715 (2017-02-12)
Notch signaling pathway is an embryonic program that becomes reactivated in pancreatic cancer and contributes to cancer stem cell (CSC) maintenance. We explored the concept of oncolytic adenoviral activity in response to Notch activation signaling, in the context of a
Kye Won Park et al.
Molecular endocrinology (Baltimore, Md.), 22(9), 2038-2048 (2008-06-20)
We previously identified the small molecule harmine as a regulator of peroxisome proliferator activated-receptor gamma (PPARgamma) and adipocyte differentiation. In an effort to identify signaling pathways mediating harmine's effects, we performed transcriptional profiling of 3T3-F442A preadipocytes. Inhibitor of DNA biding
Varsha Prakash et al.
Nature communications, 10(1), 2110-2110 (2019-05-10)
Ribosome biogenesis is a canonical hallmark of cell growth and proliferation. Here we show that execution of Epithelial-to-Mesenchymal Transition (EMT), a migratory cellular program associated with development and tumor metastasis, is fueled by upregulation of ribosome biogenesis during G1/S arrest.
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Questions

1–8 of 8 Questions  

  1. Is bovine insulin (I0516) suitable for use in rat cell culture?

    1 answer

    1. The product has not been validated for rat cell culture application. However, based on the following citations, it appears to work well with rat cell culture:

      •Isolation, Transfection, and Long-Term Culture of Adult Mouse and Rat Cardiomyocytes
      published in J Vis Exp. 2020 Oct 10;(164):10.3791/61073. PMID: 33104067.

      •Collagen hydrogels with controllable combined cues of elasticity and topography to regulate cellular processes" published in Biomed Mater. 2021 Jun 11;16(4). PMID: 34030146.

      Helpful?

  2. Does this solution contain zinc? How would that affect how quickly the insulin would act in a mammalian cell culture?

    1 answer

    1. The product contains 10 mg/mL insulin in 25 mM HEPES, pH 8.2, but it does not include zinc. Currently, the effect of zinc on insulin signaling is not characterized. However, it is noted that zinc appears to be able to enhance insulin-mediated IR tyrosine phosphorylation according to the article Biol Trace Elem Res. 2004 Oct;101(1):19-36. Effect of chromium and zinc on insulin signaling in skeletal muscle cells. PMID: 15516700.

      Helpful?

  3. is this product suitable for 3T3-L1 differentiation?

    1 answer

    1. This product is supplied as a sterile bovine insulin solution at 10 mg/mL and would be suitable for diffentiation assays. This item has not been tested on 3T3-L1 cells, but is part of the differentiation protocol for the WT-1 preadipocyte cell line, product SCC255. See the link below to review this protocol:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/568/334/scc255ds.pdf

      Helpful?

  4. How many units/mg am I getting in Product I0516, Insulin solution?

    1 answer

    1. This solution is made using insulin Product No. I5500.  The potency specification for the I5500 is not less than 27 USP units/mg.  However, we do not test for potency of the final product of this solution.  It is possible some activity is lost during the manufacturing process.

      Helpful?

  5. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  6. What is the amino acid sequence for insulin?

    1 answer

    1. The sequence and structure of insulin can be found on the product information sheet (under Documents, above).

      Helpful?

  7. What is the molarity of Product I0516, Insulin solution?

    1 answer

    1. Product No. I0516 is a 10 mg/mL solution of bovine insulin (MW 5733.5). The molarity is 1.7 μmol/mL or 1.7 mM.

      Helpful?

  8. What is the comparison between International Units (IU) and USP units for insulin?

    1 answer

    1. The international unit (IU) of insulin is based on the International Standard Preparation of Bioassay (1958). The standard preparation is a mixture of 52% bovine and 48% porcine insulin, containing 24 units/mg. Reference: Martindale: The Extra Pharmacopoeia, 29th ed., p. 392. High purity bovine pancreatic insulin typically assays at approximately 27 USP units per mg. So the IU and USP unit are not identical, but they are approximately 1:1.

      Helpful?

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