Hyaluronidase is soluble in water, however most aqueous buffers will be suitable. For testing purposes the bovine enzyme is reconstituted in 20 mM sodium phosphate buffer, with 77 mM sodium chloride, 0.01% BSA, pH 7.0. Solutions are stable up to 1 month refrigerated at 4 deg. C. For long term storage, maintain a concentration of >1 mg/mL, aliquot and freese at - 20 deg. C. Avoid repeated freeze/thaw cycles.
H6254
Hyaluronidase from sheep testes
Type V, lyophilized powder, ≥1,500 units/mg solid
Sinonimo/i:
Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
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500 MG
CHF 566.00
1 G
CHF 1’040.00
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Cambia visualizzazione
500 MG
CHF 566.00
1 G
CHF 1’040.00
About This Item
Numero CAS:
Numero CE:
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54
Prodotti consigliati
Origine biologica
sheep testis
Livello qualitativo
Tipo
Type V
Stato
lyophilized powder
Attività specifica
≥1,500 units/mg solid
PM
55 kDa
applicazioni
diagnostic assay manufacturing
Temperatura di conservazione
−20°C
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Descrizione generale
Applicazioni
Hyaluronidase has been used:
- as a component of enzyme mix for the isolation of sertoli and germ cells from seminiferous tubules[3]
- in the pre-treatment of deparaffinized adenomas sections for immunohistochemistry[4]
- in the digestion of umbilical cord blood vessel for the generation of umbilical cord stromal stem cell lines[5]
Azioni biochim/fisiol
Hyaluronidase from sheep testis is commercially used to depolymerise hyaluronic acid and chondroitin-sulphate in connective tissue and cartilage respectively.[1]
These enzymes randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.
Definizione di unità
One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).
Altre note
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Resp. Sens. 1
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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I clienti hanno visto anche
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Articular cartilage of the knee joint is avascular, exists under a low oxygen tension microenvironment, and does not self-heal when injured. Human infrapatellar fat pad-sourced mesenchymal stem cells (IFP-MSC) are an arthroscopically accessible source of mesenchymal stem cells (MSC) for
Structure of human hyaluronidase-1, a hyaluronan hydrolyzing enzyme involved in tumor growth and angiogenesis
Chao KL, et al.
Biochemistry, 46(23), 6911-6920 (2007)
Isolation of Sertoli cells from adult rat testes: an approach to ex vivo studies of Sertoli cell function
Anway MD, et al.
Biology of Reproduction, 68(3), 996-1002 (2003)
Human umbilical cord stromal stem cell express CD10 and exert contractile properties
Farias VA, et al.
Placenta, 32(1), 86-95 (2011)
Articoli
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
Protocolli
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
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