Unfortunately, the solution stability of this material at 2-8°C has not been determined. However, solutions may be stored in aliquots at -20°C for up to 3 months.
H2625
Hemoglobin from bovine blood
suitable for protease substrate, substrate powder
Sinonimo/i:
Bovine hemoglobin, Hb, Methemoglobin
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25 G
CHF 90.80
100 G
CHF 318.00
1 KG
CHF 1’520.00
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Cambia visualizzazione
25 G
CHF 90.80
100 G
CHF 318.00
1 KG
CHF 1’520.00
About This Item
Prodotti consigliati
Origine biologica
bovine blood
Stato
substrate powder
PM
Mr ~64500
tecniche
activity assay: suitable
Solubilità
H2O: soluble 20 mg/mL
Compatibilità
suitable for protease substrate
Temperatura di conservazione
2-8°C
Informazioni sul gene
cow ... HBA1(281221) , HBB(280813) , HBE2(513240) , HBE4(513108) , HBG(511735) , alpha globin(512439)
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Descrizione generale
Hemoglobin is the major component of red blood cells and is responsible for their red color. Its normal concentration in erythrocytes is 34%.[1] Hemoglobin is a globular protein with α and β chains with every 141 and 146 amino acids respectively. It exists as a tetramer with each monomer having heterocyclic porphyrin ring with iron constituting the heme.[2]
Applicazioni
Hemoglobin from bovine blood has been used as a substrate in cathepsin D activity assay.[3][4] It has also been used to measure the activity of acid proteases (pepsin-like) in the stomach extract.[5]
The solubility of α-elastin has been applied to construction of elastin-mimetic biomaterials.
Azioni biochim/fisiol
Bovine hemoglobin is used in the production of hemoglobin-vesicles (HbV). It has high thermal stability and high oxygen affinity when compared to human hemoglobin.[6] Bovine hemoglobin interacts with synthetic and azo dyes.[2] Polymerized forms of bovine hemoglobin are used to treat autoimmune hemolytic anemia.[7]
Oxygen transporter, NO scavenger
Oxygen transporter. The Fe2+/Fe3+ balance is a physiological indicator of blood oxygenation; deoxygenated hemoglobin accessorizes a feedback loop by reducing nitrite to NO, a vasodilator which enhances blood flow to oxygen-deprived tissues.
Avvertenza
Since native hemoglobin is readily oxidized in air, these preparations may be predominantly methemoglobin.
Nota sulla preparazione
Prepared from washed, lysed and dialyzed erythrocytes.
Applicazioni
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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I clienti hanno visto anche
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Journal of the American Society for Mass Spectrometry, 20(3), 484-495 (2008-12-23)
The conformations of gas-phase ions of hemoglobin, and its dimer and monomer subunits have been studied with H/D exchange and cross section measurements. During the H/D exchange measurements, tetramers undergo slow dissociation to dimers, and dimers to monomers, but this
A study of the interaction of bovine hemoglobin with synthetic dyes using spectroscopic techniques and molecular docking
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Protocolli
This procedure may be used for determination of Pepsin activity using hemoglobin as the substrate. It is a spectrophotometric stop rate determination.
Proteinase K activity measured via spectrophotometry using hemoglobin substrate, crucial for enzyme characterization.
Chromatograms
application for HPLC-
If made up in solution and stored at 2-8C, how long can this product last for?
1 answer-
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Is this product suitable for mammalian cell culture?
1 answer-
H2625 is specified to be suitable as a substrate in a protease assay. It is not evaluated for cell culture use.
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Do you have any information on purity of this product?
1 answer-
This product has not been tested for purity. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/400/152/h2500pis.pdfHelpful?
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