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T0699

Sigma-Aldrich

Trichloroacetic acid solution

6.1 N

Sinonimo/i:

TCA

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About This Item

Formula condensata:
Cl3CCOOH
Numero CAS:
76-03-9
Peso molecolare:
163.39
Beilstein:
970119
Numero MDL:
MFCD00004177
Codice UNSPSC:
12352106
ID PubChem:
57654645
NACRES:
NA.26

Forma fisica

liquid

Concentrazione

6.1 N
~100 % (w/v)

Stringa SMILE

OC(=O)C(Cl)(Cl)Cl

InChI

1S/C2HCl3O2/c3-2(4,5)1(6)7/h(H,6,7)
YNJBWRMUSHSURL-UHFFFAOYSA-N

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Descrizione generale

Trichloroacetic acid (TCA) is a strong acid. At the pH of drinking water, TCA exists almost in salt form.

Applicazioni

Trichloroacetic acid solution has been used:
  • in indoleamine 2,3-dioxygenase (IDO) enzyme assay to hydrolyze N-formylkynurenine and produce kynurenine
  • in the proliferation of human pulmonary artery smooth muscle cells (HPASMCs)
  • to treat ground tissue and precipitate proteins during protein extraction and quantification

Azioni biochim/fisiol

Trichloroacetic acid solution is traditionally used to precipitate protein. It can be used to determine protein concentration by quantitative precipitation. Trichloroacetic acid can also be used as a decalcifier and fixative in microscopy.
Trichloroacetic acid (TCA) with no known systemic toxicity, is used as a time-honored agent for superficial peeling. It is a peroxisome proliferator.

Avvertenze

Danger

Indicazioni di pericolo

H314,H335,H410

Classi di pericolo

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

8A - Combustible corrosive hazardous materials

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

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Protocolli

Enzymatic Activity of Glucose-6-Phosphatase [EC 3.1.3.9]

To measure glucose-6-phosphatase activity, the Taussky-Shorr method is used. This method is a spectrophotometric stop-rate determination assay that is measured at 660 nm.

Enzymatic Assay of Pepsin (3.4.23.1)

This procedure may be used for determination of Pepsin activity using hemoglobin as the substrate. It is a spectrophotometric stop rate determination.

Enzymatic Assay of Amyloglucosidase (EC 3.2.1.3)

This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.

Proteinase K Enzymatic Assay

Proteinase K activity measured via spectrophotometry using hemoglobin substrate, crucial for enzyme characterization.

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