Passa al contenuto
Merck
Tutte le immagini(1)

Documenti fondamentali

F4135

Sigma-Aldrich

Siero fetale bovino

Fetal Bovine Serum

USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for insect cell culture, suitable for hybridoma

Sinonimo/i:

FBS HI (inattivato col calore), FCS HI (inattivato con calore), Sieri HI (inattivati col calore), Siero HI (inattivato col calore), FBS, FCS, sieri, siero

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Tutte le immagini(1)

About This Item

Numero MDL:
MFCD00132239
Codice UNSPSC:
12352207
eCl@ss:
32160409
NACRES:
NA.71
Origine biologica:
bovine fetus
Origine:
USA origin
Sterilità:
sterile-filtered
Condizioni di spedizione:
dry ice
Prezzi e disponibilità al momento non sono disponibili

Origine biologica

bovine fetus

Livello qualitativo

500

Sterilità

sterile-filtered

Nome Commerciale

FBS Classic

Composizione

Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL

Origine

USA origin

tecniche

cell culture | hybridoma: suitable
cell culture | insect: suitable
cell culture | mammalian: suitable

Impurezze

≤10 EU/mL endotoxin

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Cerchi prodotti simili? Visita Guida al confronto tra prodotti

Descrizione generale

Il siero fetale bovino (FBS) è un supplemento dei terreni molto utilizzato in quanto è in grado di svolgere una vasta gamma di funzioni nelle colture cellulari. L′FBS fornisce nutrienti, fattori di crescita e di adesione e contribuisce alla proliferazione.[1] Protegge inoltre le cellule dal danno ossidativo e dall′apoptosi.[2] L′FBS blocca la generazione delle specie reattive dell′ossigeno, che altrimenti non vengono eliminate nei sistemi di terreni privi di siero (SFM).[3]

Applicazioni

L′FBS proveniente dagli Stati Uniti è utilizzato in un′ampia gamma di applicazioni per colture cellulari. L′aggiunta di FBS al terreno apporta molti fattori non definiti che promuovono la proliferazione e la sopravvivenza delle cellule in coltura.
Il siero fetale bovino è stato usato:
  • come componente del Dulbecco′s Modified Eagles Media (DME, DMEM),[4] Eagle′s Minimum Essential Medium (MEM); (EMEM),[5] Roswell Park Memorial Institute medium (RPMI-1640),[6] α-modified eagle medium (αMEM) (MEM)[7] e Leibovitz L-15 medium[8]
  • come componente del terreno di bloccaggio nel trattamento di sezioni di tronco encefalico[9]
  • per migliorare l′adesione degli espianti dermici[10]

Nota sulla preparazione

Preparato per riscaldamento a 56 °C per 30 minuti.
Questo prodotto viene preparato mediante riscaldamento a 56 °C per 30 minuti. Il riscaldamento del siero a 56 °C per 30 minuti inattiva il sistema del complemento per i test immunologici. L'attivazione col calore ha anche lo scopo di inattivare altri eventuali inibitori della crescita cellulare in coltura.

Risultati analitici

  • Esaminato per la presenza di batteri, virus e micoplasmi
  • Sottoposto a tripla filtrazione con membrana da 0,1 micron in condizioni asettiche.

  • Testato per il contenuto in endotossine ed emoglobina

Comunemente ordinati con questo prodotto

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves

Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number
23D340
23B289
19C547H1-A
22L538
22G096

Non trovi la versione di tuo interesse?

Se hai bisogno di una versione specifica, puoi cercare il certificato tramite il numero di lotto.

Cerca un COA

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

I clienti hanno visto anche

Slide 1 of 2

1 of 2

Gentamicina 50 mg/mL in deionized water, liquid, 0.1 μm filtered, BioReagent, suitable for cell culture

Sigma-Aldrich

G1397

Gentamicina

SAFC

SAFC

55037C

Hanks′ Balanced Salt solution

Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts: an in vitro approach to bovine digital dermatitis
Akkoc A, et al.
Journal of Applied Animal Research, 46, 445-450 (2018)
Combining probiotic Phaeobacter inhibens DSM17395 and broad-host-range vibriophage KVP40 against fish pathogenic vibrios
Rasmussen BB, et al
Aquaculture (Amsterdam, Netherlands), 513, 734415-734415 (2019)
Antileishmanial activity study and theoretical calculations for 4-amino-1, 2, 4-triazole derivatives
Suleymanouglu N, et al.
Journal of Molecular Structure, 1144, 80-86 (2017)
Ming Guan et al.
American journal of translational research, 11(3), 1343-1358 (2019-04-12)
Aging and exposure to noise or ototoxic drugs are major causes of hair cell death leading to human hearing loss, and many agents have been developed to protect hair cells from apoptosis. Fetal bovine serum (FBS) is a fundamental ingredient
Sanjay Ghosh et al.
Nucleic acids research, 44(9), e84-e84 (2016-02-07)
Long non-coding RNAs (lncRNAs) have emerged as regulators of gene expression across metazoa. Interestingly, some lncRNAs function independently of their transcripts - the transcription of the lncRNA locus itself affects target genes. However, current methods of loss-of-function analysis are insufficient
Visualizza tutti i documenti correlati

Articoli

Calcium in Cell Culture

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

Fetal Bovine Serum (FBS) – What You Need to Know

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

Serum for Cell and Tissue Culture FAQs

Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.

Protocolli

Freezing and Thawing FBS and Other Sera

Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.

Contenuto correlato

Evaluating of Stericup® Quick Release Filtration Devices for Fetal Bovine Serum (FBS) in Cell Culture

Learn how to effectively filter fetal bovine serum (FBS) using Stericup® Quick Release vacuum filtration devices, including flow test data and analysis.

Fetal Bovine Serum that Makes the Grade

Explore our portfolio of fetal bovine serum (FBS) and determine the right FBS for your lab needs and budget. Request a serum sample.

Siero fetale bovino sempre all’altezza

Mettiamo a vostra disposizione un assortimento completo di sieri fetali bovini (FBS). Per identificare quello più adatto alle esigenze e al budget del vostro laboratorio, non esitate a chiedere un campione.

Mpox qPCR Products

Products and support for mpox research, offering world-class reagents for immunoassay and molecular assay development.

1 Review This action will navigate to reviews.

Questions

1–6 of 6 Questions  

  1. Is it possible to store the FBS at a temperature of -80°C, which is lower than the recommended storage temperature of -20°C?

    1 answer

    1. The FBS should be stored at the recommended temperature of -20°C. Storage at -80°C has not been tested, but there are no anticipated issues. Frost Free Freezers should be avoided for storage.

      Helpful?

  2. Recommended storage is listed as -20C; is it safe/ok to store at -80C?

    1 answer

    1. The recommended long-term storage temperature for FBS is -20C. For the short-term, such as for shipping, packing on dry ice at -40C is recommended. It is not recommended to store the FBS at temperatures lower than -40C. Please see the guide on freezing and thawing FBS and other sera below:
      https://www.sigmaaldrich.com/technical-documents/protocol/cell-culture-and-cell-culture-analysis/cell-culture-media-preparation/storing-and-thawing-serum

      Helpful?

  3. Once a bottle of Fetal Bovine Serum (FBS) is thawed, for how long can the bottle be used?

    1 answer

    1. Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored  refrigerated at 2-8°C.  If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days.  For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance.  For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C.  For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  5. Why is serum Heat-Inactivated (HI)?

    1 answer

    1. Heat inactivation of serum is performed to inactivate complement (which can cause cell lysis or downstream problems in immunoassays). If there are immunoglobulins present in the serum that can bind to the cells in culture (either specifically or non-specifically), there is a chance for antibody-mediated complement cytotoxicity (ADCC, causing cell death). It may also reduce the titer of some mycoplasma and some viruses. Often, heat-inactivated serum is used because of the adoption of a previous protocol. It is mainly used when culturing hematopoetic cells or performing immunological type assays such as mixed-lymphocyte reactions or neutralization assays.FBS may not be heat inactivated as this is not required for all applications. It limits the loss of the other protein activity that may be minimally present in the serum.A protocol for heat inactivation:1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Sigma-Aldrich does not recommend repeated thaw-freeze cycles.

      Helpful?

  6. Does Sigma-Aldrich offer delipidated bovine serum?

    1 answer

    1. At this time, Sigma-Aldrich does not offer delipidated bovine serum (either FBS or calf serum).A method for lipid depletion, though not necessarily that of Sigma:1. While mixing, gradually add 10 g fumed silica (Product S5130 or Aerosil) per liter of serum2. Mix at room temperature and then transfer to 2-8 °C for overnight mixing.3. After overnight mixing, remove flumed silica (Aerosil) by centrifugation at 3000-5000 rpm for 5 minutes.4. Retain the supernatant. The lipids should stick to the silica. There may be some protein loss

      Helpful?

Reviews

Active Filters

  1. 1 Ratings-Only Review

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.