This product is a liquid medium. The density is not reported. The 1L volume weighs approximately 1 kilogram. The total weight of the packaged 1L size is 1.1 kg. The total weight of the packaged 500 mL size is 578 g.
D6429
DMEM - high glucose
With sodium bicarbonate, ʟ-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture
Dulbecco’s Modified Eagle’s Medium - high glucose
1 of 5 reviewers received a sample product or took part in a promotion
Sinonimo/i:
DME, DMEM
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Informazioni su questo articolo
UNSPSC Code:
12352207
NACRES:
NA.71
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Terreno Eagle modificato di Dulbecco - alto glucosio, With 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
HEPES: no
NaHCO3: yes
phenol red: yes
sodium pyruvate: yes
L-glutamine: yes
glucose: high
shipped in
ambient
storage temp.
2-8°C
Quality Level
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Questo articolo | D6546 | D5796 | D1145 |
|---|---|---|---|
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| form liquid | form liquid | form liquid | form liquid |
| components HEPES: no | components NaHCO3: yes | components NaHCO3: yes | components L-glutamine: no |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
General description
Questo terreno di coltura DMEM-Hi ad alto glucosio è un terreno 1x completo arricchito con piruvato di sodio. Si differenzia dalla formulazione DMEM-Hi originale in quanto la contiene piridossina al posto del piridossale. Il piridossale è un componente instabile dei terreni di coltura.
Application
Il terreno Eagle modificato di Dulbecco (DMEM) è una versione modificata del terreno basale Eagle (BME) che contiene concentrazioni quattro volte superiori di amminoacidi e vitamine. La formulazione originale conteneva 1000 mg/L di glucosio ed è stata utilizzata per coltivare cellule embrionali di topo. Da allora, il terreno è stato modificato in diversi modi per favorire le colture primarie di cellule di topo e pollo e di molte altre cellule normali e trasformate. Ognuno di questi terreni offre una diversa combinazione di L-glutammina e piruvato di sodio. Inoltre, i livelli di glucosio sono stati innalzati a 4500 mg/L, motivo per cui il terreno è denominato “DMEM/High”.
Related product
N° Catalogo
Descrizione
Determinazione del prezzo
Classe di stoccaggio
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Instructions
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How much does D6429-1L weigh? Thank you!
1 answer-
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Hi, What is the concentration of endotoxin detected in DMEM high glucose media D6429?
1 answer-
This product has an endotoxin specification of ≤ 1 EU/ml or 0.1 ng/mL. The lot specific values are reported in the Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/d6429#product-documentationHelpful?
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What is the concentration of Sodium Bicarbonate and Sodium Pyruvate in it?
1 answer-
This formulation contains 3.7 g/L sodium bicarbonate and 110 mg/L sodium pyruvate. The composition of this product is published on page 3 of the product information sheet. Please see the link below to access this document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/318/256/d6429for.pdf
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After opening a bottle of medium: how long can I use this bottle ?(stored at 4C°)
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Provided that the product is handled and stored properly and has not been contaminated, the medium will be stable up to the expiration date. This date is provided on the label and the lot-specific Certificate of Analysis.
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I would like to inquire about the amino acid formulation in DMEM (#6429, #6546). Specifically, could you provide information on the concentration of aspartate and glutamate in these formulations? Thank you very much.
1 answer-
The composition of this product is published on page 3 of the product information sheet. Please see the link below to access this document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/318/256/d6429for.pdf
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