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E2884

Sigma-Aldrich

Esterase from porcine liver

ammonium sulfate suspension, ≥150 units/mg protein (biuret)

Sinonimo/i:

Carboxyl esterase, Carboxylic-ester hydrolase, PLE

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200 UNITS
CHF 76.20
1000 UNITS
CHF 102.00
5000 UNITS
CHF 213.00
20000 UNITS
CHF 644.00

CHF 76.20

Spedizione prevista il05 aprile 2025

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200 UNITS
CHF 76.20
1000 UNITS
CHF 102.00
5000 UNITS
CHF 213.00
20000 UNITS
CHF 644.00

About This Item

Numero CAS:
9016-18-6
Classificazione EC (Enzyme Commission):
3.1.1.1 (BRENDA, IUBMB)
Numero MDL:
MFCD00131030
Codice UNSPSC:
12352204
NACRES:
NA.54

CHF 76.20

Spedizione prevista il05 aprile 2025

Origine biologica

Porcine liver

Livello qualitativo

300

Stato

ammonium sulfate suspension

Attività specifica

≥150 units/mg protein (biuret)

PM

168 kDa

Temperatura di conservazione

2-8°C

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Descrizione generale

Esterase, also known as carboxyl ester hydrolase[1] is a member of the hydrolase superfamily of enzymes.[2]

Applicazioni

Esterase from porcine liver has been used to unmask the fluorescence in ultraviolet-visible spectroscopy.[3]
Porcine liver esterase is used to catalyze the hydrolysis of pentaacetyl catechin and epicatechin for use in pharmaceutical and industrial applications.

Pig liver esterase is commonly used for kinetic resolutions and assymetric synthesis in organic chemistry.
The enzyme from Sigma has been used to develop an alternative gel-based protocol for screening for bead-bound catalytic activity in aqueous media.[4] The product has also been used to investigate the properties of the compound L-valine-3-{8-[(E)-2-[3-methoxyphenyl)ethenyl]-7-methyl-1-propargylxanthine-3-yl}propyl ester hydrochloride (MSX-4, an aminoacid ester prodrug of the adenosine A2A receptor antagonist MSX-2).[5]

Azioni biochim/fisiol

Esterase acts on water-soluble carboxyl esters containing short chain fatty acids. Its functionality is attributed to the catalytic triad of Ser, His and Asp/Glu.
Esterase enzyme of bacteria and fungi seems to have several industrial applications.[2] It is capable of catalyzing the hydrolysis and synthesis of ester bonds.[1]
Pig liver esterase catalyzes enantioselective conversion of an ester to a carboxylic acid. The molecular weight is found to be 168 kDa. It is a serine enzyme with two active sites on each molecule, which dissociates into active half-molecules in the presence of dilute acid or concentrated salts.[6]

Definizione di unità

One unit will hydrolyze 1.0 μmole of ethyl butyrate to butyric acid and ethanol per min at pH 8.0 at 25 °C.

Stato fisico

Suspension in 3.2 M (NH4)2SO4, pH 8

Substrato

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificati d'analisi (COA)

Lot/Batch Number
0000366726
0000355126
0000352049
0000352073
0000310637

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With the discovery of an increasing number of biologically active peptides and peptide mimetics (1-3), there is a pressing need for the development of strategies to deliver these biologically active compounds to the desired site of action. The preceding two
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Articoli

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Protocolli

Enzymatic Assay of Esterase

Objective: To standardize a procedure for the enzymatic determination of Esterase activity using Ethyl Butyrate as a substrate.

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