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E0887

Esterase from rabbit liver

lyophilized powder, ≥30 units/mg protein

Sinonimo/i:

Carboxyl esterase, Carboxylic-ester hydrolase

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A voi/SKUDisponibilitàPrezzo
500 units
Per conoscere la disponibilità, visualizza il carrello
CHF 239.00
1000 units
Per conoscere la disponibilità, visualizza il carrello
CHF 397.00

Informazioni su questo articolo

Numero CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-773-7
MDL number:
Numero CE:
Specific activity:
≥30 units/mg protein

CHF 239.00


Per conoscere la disponibilità, visualizza il carrello

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form

lyophilized powder

Quality Segment

specific activity

≥30 units/mg protein

mol wt

60 kDa

composition

Protein, ≥85% biuret

storage temp.

−20°C

General description

Esterase belongs to the C-family of carboxylesterases. It is a glycoprotein, mainly present in the endoplasmic reticulum of hepatocytes.

Application

The enzyme from Sigma has been used to study the effect of divalent metal ions on the activity of esterase.
Esterase from rabbit liver has been used:
  • in comparative biophysical studies with bovine bile-salt-activated lipase in the assays with vinyl acetate, triacylglycerols, p-nitrophenyl esters and serine enzyme inhibitors
  • in the catalysis and kinetic profiling studies of peptidic compounds
  • in in vitro stability assays of prodrug compounds (BMS-292655 and BMS300043)
  • in the hydrolysis of oleuropein aglycone (Ole-AG)

Esterase from rabbit liver has been used in a study to investigate a toxic effect of carbamate insecticides. Esterase from rabbit liver has also been used in a study to investigate the effect of simvastatin on expression and activity of a lipoprotein-associated phospholipase A(2).

Biochem/physiol Actions

Esterase enzyme catalyzes hydrolysis of carboxylic ester to carboxylate and alcohol in the presence of water. Rabbit liver carboxylesterase catalyzes enantioselective hydrolysis of cineol derivatives.
Esterase enzyme has broad substrate specificity. Esterase from rabbit liver has been used to investigate the toxic effect of carbamate insecticides. Esterase from rabbit liver has also been used for the hydrolysis of simvastatin.

Physical form

Lyophilized powder containing Tris buffer salts

Other Notes

One unit will hydrolyze 1.0 μmole of ethyl butyrate to butyric acid and ethanol per min at pH 8.0 at 25 °C.

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Questo articolo
E2884E301943388
description

lyophilized powder, ≥30 units/mg protein

description

ammonium sulfate suspension, ≥150 units/mg protein (biuret)

description

lyophilized powder, ≥15 units/mg solid

description

recombinant, expressed in E. coli, ≥30.0 U/g

specific activity

≥30 units/mg protein

specific activity

≥150 units/mg protein (biuret)

specific activity

≥15 units/mg solid

specific activity

≥30.0 U/g

form

lyophilized powder

form

ammonium sulfate suspension

form

lyophilized powder

form

crystalline, crystals, powder or flakes

mol wt

60 kDa

mol wt

168 kDa

mol wt

168 kDa

mol wt

-

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

300

Quality Level

300

Quality Level

100

composition

Protein, ≥85% biuret

composition

-

composition

-

composition

-


pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Classe di stoccaggio

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Questions

  1. Is it possible for the esterase from rabbit liver (E0887) to hydrolyze ethyl hexanoate into hexanoic acid?

    1 answer
    1. The product has been evaluated for the conversion of ethyl butyrate to butyric acid and ethanol. However, there has been no evaluation for the hydrolysis of ethyl hexanoate into hexanoic acid, and there are no known citations of this product for ethyl hexanoate hydrolysis. Due to the structural similarity between ethyl hexanoate and ethyl butyrate, both containing an ester bond, it's possible that this product could hydrolyze ethyl hexanoate, as esterases typically accommodate a wide range of ester substrates. However, the rate and efficiency of the hydrolysis may differ from the enzyme activity tested using ethyl butyrate as a substrate.

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