DUO92020
Duolink® In Situ PLA® Probe Anti-Human PLUS
Affinity purified Donkey anti-Human IgG (H+L)
Sinonimo/i:
in situ Proximity Ligation Assay, Protein Protein Interaction Kit
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About This Item
Prodotti consigliati
Origine biologica
donkey (Polyclonal)
Forma dell’anticorpo
affinity purified immunoglobulin (Secondary antibody)
Nome Commerciale
Duolink®
Reattività contro le specie
human
tecniche
immunofluorescence: suitable
proximity ligation assay: suitable
Compatibilità
suitable for brightfield
suitable for fluorescence
Condizioni di spedizione
wet ice
Temperatura di conservazione
2-8°C
Categorie correlate
1 of 4
Questo articolo | DUO92021 | DUO92010 | DUO92009 |
|---|---|---|---|
| technique(s) immunofluorescence: suitable, proximity ligation assay: suitable | technique(s) immunofluorescence: suitable, proximity ligation assay: suitable | technique(s) immunofluorescence: suitable, proximity ligation assay: suitable | technique(s) immunofluorescence: suitable, proximity ligation assay: suitable |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| product line Duolink® | product line Duolink® | product line Duolink® | product line Duolink® |
| biological source donkey (Polyclonal) | biological source donkey (Polyclonal) | biological source - | biological source - |
Applicazioni
This product can be applied to both the Duolink® In Situ Fluorescence Protocol and the Duolink® In Situ Brightfield Protocol depending on the detection reagents used.
Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting and more.
To perform a complete Duolink® PLA® in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.HRP is also available for brightfield detection.
PLA probe anti-Human reacts with whole molecule human IgG and the light chains of other human immunoglobulin?s. The PLA Probe Anti-Human Plus has minimal cross-reactivity with bovine, chicken, goat, guinea pig, Syrian hamster, horse, mouse, rabbit, rat, and sheep serum proteins. A MINUS probe of a different species must be used simultaneously with this product. See our Product Selection Guide for more information.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects
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Caratteristiche e vantaggi
- No overexpression or genetic manipulation required
- High specificity (fewer false positives)
- Single molecule sensitivity due to rolling circle amplification
- Relative quantification possible
- No special equipment needed
- Quicker and simpler than FRET
- Increased accuracy compared to co-IP
- Publication-ready results
Componenti
- 5x PLA Probe Anti-Human PLUS - Donkey anti-human secondary antibody conjugated to oligonucleotide PLUS
- 1x Blocking Solution - Reagent for blocking of the sample
- 1x Antibody Diluent - For dilution of PLA probes and primary antibodies
See datasheet for more information.
Note legali
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Aquatic Chronic 2 - Skin Sens. 1
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
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Articoli
Support information including tips and tricks, frequently asked questions, and basic troubleshooting.
Things to consider for preparation, setup and execution of the Duolink® assay protocol
Contenuto correlato
Applications to detect, quantify and visualize protein-protein interactions, post-translational modifications and low expression protein detection using proximity ligation assay
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