11681451001
Roche
NBT/BCIP Stock Solution
Sinonimo/i:
5-bromo-4-chloro-3-indoyl phosphate, BCIP
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Codice UNSPSC:
12352204
Prodotti consigliati
Forma fisica
solution
Livello qualitativo
PM
817.7
Confezionamento
pkg of 8 mL
Produttore/marchio commerciale
Roche
Temperatura di conservazione
2-8°C
Descrizione generale
The reaction product has a blue color and is insoluble in water. The blue color can be visually seen.
Formulas: NBT: C40H30Cl2N10O6, BCIP: C8H6NO4BrClP x C7H9N
Evaluation: visually
Formulas: NBT: C40H30Cl2N10O6, BCIP: C8H6NO4BrClP x C7H9N
Evaluation: visually
Applicazioni
- The solution is used for the sensitive detection of alkaline phosphatase (AP) in blotting protocols, including: Southern blot
- Northern blot
- Western blot
- Colony and plaque lifts
- In situ hybridization
- and for applications in immunohistochemistry and immunocytochemistry.
Stato fisico
Solution of 18.75 mg/ml nitro blue tetrazolium chloride and 9.4 mg/ml 5-bromo-4-chloro-3-indolyl-phosphate, toluidine-salt in 67% (DMSO) (v/v).
Note: The color of the solution can vary from yellowish to light brown.
Note: The color of the solution can vary from yellowish to light brown.
Nota sulla preparazione
Working solution: Preparation of staining solution
Always prepare this solution fresh shortly before use.
Always prepare this solution fresh shortly before use.
- For all applications except DIG System: Add 200 μl of the stock solution to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl, 0.05 M MgCl2.
- For DIG System applications: Add 200 μl of the stock solution to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl
Altre note
For life science research only. Not for use in diagnostic procedures.
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
does not flash
Punto d’infiammabilità (°C)
does not flash
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
I clienti hanno visto anche
Jaroslav Fabik et al.
Biology open, 9(6) (2020-07-04)
Cranial neural crest cells (cNCCs) originate in the anterior neural tube and populate pharyngeal arches in which they contribute to formation of bone and cartilage. This cell population also provides molecular signals for the development of tissues of non-neural crest
João L Q Durigan et al.
Muscle & nerve, 49(1), 120-128 (2013-04-30)
Neuromuscular electrical stimulation (NMES) is used to improve quadriceps mass after anterior cruciate ligament (ACL) injury. We studied the effect of NMES on mRNA levels of atrophy genes in the quadriceps muscle of rats after ACL transection. mRNA levels of
Garth R Ilsley et al.
Scientific reports, 10(1), 4961-4961 (2020-03-20)
Single-cell RNA-seq has been established as a reliable and accessible technique enabling new types of analyses, such as identifying cell types and studying spatial and temporal gene expression variation and change at single-cell resolution. Recently, single-cell RNA-seq has been applied
David Lagman et al.
PloS one, 10(3), e0121330-e0121330 (2015-03-26)
Gene duplications provide raw materials that can be selected for functional adaptations by evolutionary mechanisms. We describe here the results of 350 million years of evolution of three functionally related gene families: the alpha, beta and gamma subunits of transducins
Rebecca McLennan et al.
Developmental biology, 339(1), 114-125 (2009-12-29)
The neural crest is an excellent model to study embryonic cell migration, since cell behaviors can be studied in vivo with advanced optical imaging and molecular intervention. What is unclear is how molecular signals direct neural crest cell (NCC) migration
Protocolli
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.