11363514910
Roche
DIG Luminescent Detection Kit
sufficient for 50 blots (10 cm x 10 cm each), kit of 1 (5 components), suitable for hybridization
Sinonimo/i:
luminescent detection kit
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About This Item
Codice UNSPSC:
41116134
Prodotti consigliati
Saggio
>98% (NMR)
Livello qualitativo
impiego
sufficient for 50 blots (10 cm x 10 cm each)
Confezionamento
kit of 1 (5 components)
Produttore/marchio commerciale
Roche
Caratteristiche più verdi
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
tecniche
hybridization: suitable
Categoria alternativa più verde
, Aligned
Temperatura di conservazione
−20°C
Descrizione generale
DIG Luminescent Detection Kit is used for the detection of digoxigenin-labeled nucleic acids by enzyme immunoassay with luminescence on nylon membranes. The nonradioactive DIG system uses digoxigenin, a steroid hapten, coupled to deoxyuridine triphosphate (dUTP), UTP or didUTP to label DNA, RNA or oligonucleotides for hybridization and subsequent luminescent detection.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Applicazioni
DIG Luminescent Detection Kit has been used for highly sensitive and specific detection of DIG-labeled nucleic acids in:
- critical commercial assays
- Southern hybridization
- northern analysis
- plaque or colony lifts with anti-DIG-AP conjugate and the chemiluminescent substrate CSPD. Chemiluminescent detection with CSPD (following the instructions of the kit) is as sensitive as radioactive methods, but requires much shorter exposure times.
Confezionamento
1 kit containing 5 components.
Specifiche
Sensitivity: 0.03 pg of homologous DNA or 0.1 pg of homologous RNA is detected in a Southern or dot blot after <30-min exposure time. Single-copy genes are detected in 0.3 μg mammalian genomic DNA.
Nota sulla preparazione
Working concentration: 0.5mg/ml for flow cytometry
Enzymatic dephosphorylation of the dioxetane CSPD by alkaline phosphatase leads to the metastable phenolate anion, which decomposes and emits light at 477nm. The light emission increases with time until a constant intensity is attained.
Assay Time
Note: Store protected from light.
- Storage conditions (working solution): Antibody conjugate (vial 3): once opened, should be stored at 2 to 8°C
- Blocking Reagent (bottle 4): in solution at 2 to 8°C
- CSPD (vial 5): at 2 to 8°C when frequently used. Repeated freeze/thaw cycles should be avoided.
Enzymatic dephosphorylation of the dioxetane CSPD by alkaline phosphatase leads to the metastable phenolate anion, which decomposes and emits light at 477nm. The light emission increases with time until a constant intensity is attained.
Assay Time
- Immunological detection 1.5 hours
- Signal detection 5 to 30 minute
Note: Store protected from light.
Altre note
For life science research only. Not for use in diagnostic procedures.
Solo come componenti del kit
N° Catalogo
Descrizione
- DIG-labeled Control DNA 5 µg/ml
- DNA Dilution Buffer
- Anti-digoxigenin-AP antibody, Fab fragments 750 U/ml
- Blocking Reagent
- CSPD 11.6 mg/ml
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
does not flashNot applicable
Punto d’infiammabilità (°C)
does not flashNot applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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Articoli
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
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