11093657910
Roche
DIG DNA Labeling and Detection Kit
Sinonimo/i:
dna labeling and detection kit, dig
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About This Item
Codice UNSPSC:
41105500
Prodotti consigliati
impiego
sufficient for 25 labeling reactions
sufficient for 50 blots
Livello qualitativo
Produttore/marchio commerciale
Roche
Caratteristiche più verdi
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
Categoria alternativa più verde
, Aligned
Temperatura di conservazione
−20°C
Descrizione generale
DIG DNA Labeling and Detection Kit is a convenient kit for random-primed labeling of DNA with digoxigenin-deoxyuridine triphosphate (dUTP), alkali-labile and color detection of hybrids by enzyme immunoassay. In this method, the complementary strand of denatured DNA is synthesized by Klenow polymerase using the 3′-OH termini of random oligonucleotides as primers.
Contents:
Contents:
- Unlabeled Control DNA 1, 100 μg/ml
- Unlabeled Control DNA 2, 200 μg/ml
- DNA Dilution Buffer
- DIG-labeled Control DNA, 5.2 μg/ml
- 10x Hexanucleotide Mix
- 10x dNTP Labeling Mixture
- Klenow Enzyme, Labeling grade, 2 U/μl
- Anti-digoxigenin-AP-conjugate, 750 U/ml
- NBT/BCIP Concentrated Stock Solution
- Blocking Reagent
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Specificità
Sensitivity and specificity: The standard labeling reaction with 1 μg template will give a yield of 260 ng DIG-labeled DNA after a 1-hour incubation at +37°C, or 780 ng after a 20-hour incubation. The sensitivity of DIG detection depends on the concentration of the DIG-labeled probe in the hybridization reaction, and the duration of the color reaction.
Applicazioni
DIG DNA Labeling and Detection Kit has been used in a variety of hybridization techniques:
- Southern blots
- Northern blots
- Dot blots
- Colony and plaque screening
Confezionamento
1 kit containing 10 components.
Nota sulla preparazione
Working concentration: Dilution of Antibody 1:5,000
Altre note
For life science research only. Not for use in diagnostic procedures.
Solo come componenti del kit
N° Catalogo
Descrizione
- Unlabeled Control DNA 1 100 µg/ml
- Unlabeled Control DNA 2 200 µg/ml
- DNA Dilution Buffer
- DIG-labeled Control DNA 5.2 µg/ml
- Hexanucleotide Mix 10x concentrated
- dNTP Labeling Mixture 10x concentrated
- Klenow Enzyme, Labeling grade 2 U/µl
- Anti-digoxigenin-AP-conjugate antibody 750 U/ml
- NBT/BCIP Concentrated Stock Solution
- Blocking Reagent
Vedi tutto (10)
Codice della classe di stoccaggio
13 - Non Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
does not flashNot applicable
Punto d’infiammabilità (°C)
does not flashNot applicable
Certificati d'analisi (COA)
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Articoli
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
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