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SCM013

Sigma-Aldrich

NDiff Neuro-27 Medium Supplement (100x)

NDiff Neuro-27 Medium Supplement is a serum-free supplement containing antioxidants & other factors that has been developed for the in vitro propagation & maintenance of undifferentiated murine embryonic stem (ES) cells in serum free medium.

Sinonimo/i:

NDiff Medium Supplement, Neuro-27 Medium Supplement

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About This Item

Codice UNSPSC:
12352207
eCl@ss:
32160801
NACRES:
NA.75

Livello qualitativo

100

Forma fisica

liquid

Produttore/marchio commerciale

Chemicon®
Millipore

tecniche

cell culture | stem cell: suitable

input

sample type: mouse embryonic stem cell(s)

Condizioni di spedizione

dry ice

Descrizione generale

NDiff Neuro-27 Medium Supplement is a serum-free supplement containing antioxidants and other factors that has been specifically developed for the in vitro propagation and maintenance of undifferentiated murine embryonic stem (ES) cells in serum free medium (Nichols J, 2006). This product can also be used to differentiate murine ES cells into post-mitotic neurons particularly via monolayer differentiation (Ying QL, 2003).

Stato fisico

NDiff Neuro-27 Medium Supplement is supplied as an aqueous solution. Optimal working concentration of the medium supplement must be determined for specific uses and cell types. In most instances, a 1:40-1:200 dilution is appropriate. Typically, Neuro-27 is diluted 1:100 for self renewal and neural differentiation of mouse ES cells.

Stoccaggio e stabilità

Maintain at -20 °C until expiration date. Protect from light. Once thawed, aliquot unused portion and store at -20 °C until future use. Once added to the cell culture medium, the product is stable at 4 °C for four weeks.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Defined conditions for neural commitment and differentiation
Ying, Qi-Long and Smith, Austin G
Test, 365, 327-341 (2003)
Qi-Long Ying et al.
Nature biotechnology, 21(2), 183-186 (2003-01-14)
Mouse embryonic stem (ES) cells are competent for production of all fetal and adult cell types. However, the utility of ES cells as a developmental model or as a source of defined cell populations for pharmaceutical screening or transplantation is
Jennifer Nichols et al.
Methods in molecular biology (Clifton, N.J.), 329, 91-98 (2006-07-19)
The availability of murine embryonic stem (ES) cells has revolutionized the study of mammalian development and disease. We recently developed a culture medium that has enabled us to identify the essential signaling pathways required for maintenance of pluripotency in vitro.

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Protocolli

Neural Stem Cell Culture Protocols

Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.

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