This product is the native form of trypsin, beta-trypsin. This product had not been cofirmed to be cationic nor anionic isoform.
T1426
Trypsine from bovine pancreas
TPCK Treated, essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein
Synonyme(s) :
Serine Protease 1
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About This Item
Produits recommandés
Source biologique
bovine pancreas
Niveau de qualité
Qualité
Proteomics Grade
Forme
essentially salt-free, lyophilized powder
Activité spécifique
≥10,000 BAEE units/mg protein
Poids mol.
23.8 kDa
Solubilité
hydrochloric acid: soluble 1 mM
Application(s)
diagnostic assay manufacturing
Activité étrangère
Chymotrypsin ≤0.1 BTEE units/mg protein
Température de stockage
−20°C
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Application
Actions biochimiques/physiologiques
Les inhibiteurs de protéase à sérine, notamment le DFP, la TLCK, l′APMSF, l′AEBSEF et l′aprotinine, inhibent la trypsine.
Composants
Attention
Définition de l'unité
Notes préparatoires
Ce produit est également traité par TPCK et dialysé. Le traitement à la L-1-tosylamido-2-phényléthyl chlorométhyl cétone (TPCK) réduit l′activité de chymotrypsine qui est normalement présente dans la trypsine.
Inhibiteur
Substrat
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organes cibles
Respiratory system
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Équipement de protection individuelle
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Protocoles
Continuous spectrophotometric rate determination method using BAEE substrate measures trypsin activity, essential for enzyme characterization.
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Hello, we need for our experiments purified cationic trypsin. Did you ever confirm that the bovine trypsin you sell is cationic trypsin or PRSS1. Thanks
1 answer-
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I would like to ask, is the Trypsin, TPCK-treated already sterile when I solve the powder in sterile aquadest, or have I to filter the solution?
1 answer-
This product is not sterile and will need to be sterile filtered. See product SLGP33RS, Millex GP, for a suitable filter options:
https://www.sigmaaldrich.com/product/mm/slgpr33rsHelpful?
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Does Product T1426, Trypsin from bovine pancreas, work for in-gel digestions?
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For in-gel digestions, we actually recommend using a methylated trypsin. Product No. T6567 is Trypsin from porcine pancreas, proteomics grade, dimethylated. For product T6567, the lysine residues of proteomics grade trypsin have been reductively methylated, resulting in a product that is resistant to autolysis. The T6567 has also been treated with TPCK to remove chymotryptic activity, further purified through affinity chromatography, and lyophilized. This results in an enzyme that gives highly specific cleavage.
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How much of Product T1426, Trypsin from bovine pancreas, should I use to digest my peptide?
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For trypsin digestion of peptides, use a ratio (w:w) of about 1:100 to 1:20 for trypsin:peptide. Trypsin preparations usually contain some contaminating chymotrypsin and this should be inhibited with N-tosyl-L-phenylalanyl chloromethyl ketone (TPCK). Product No. T1426 has already been treated with TPCK, so it does not need to be further treated.
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What is Product T1426, Trypsin from bovine pancreas, soluble in, and how do you recommend storing solutions?
1 answer-
Trypsin is soluble in 1 mM hydrochloric acid (pH approximately 3). It is also surprisingly stable in 1 mM HCl. Solutions are stable for approximately 1 year when aliquoted and stored at -20°C. The presence of Ca2+ (20 mM) will also retard trypsin's ability to autodigest, and will therefore help to maintain the stability of the trypsin in solution.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the extinction coefficient for Product T1426, Trypsin from bovine pancreas?
1 answer-
Various values have been reported in the literature for the extinction coefficient of bovine pancreatic trypsin. The E1%(280) values have ranged from 12.9 - 15.4. When we assay the protein content of Product No. T1426 in our labs here at Sigma, we use a value of 14.4.
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