D7290
Deoxyribonucleic acid from herring sperm
single-stranded for hybridization
Synonyme(s) :
DNA
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About This Item
Numéro CAS:
Numéro CE :
Numéro MDL:
Code UNSPSC :
41106310
eCl@ss :
32160414
Nomenclature NACRES :
NA.52
Produits recommandés
Source biologique
(herring sperm)
Qualité
for molecular biology
Forme
solution
Conditions d'expédition
dry ice
Température de stockage
−20°C
Description générale
Suitable for use as a blocking agent in Southern hybridizations.
Application
Deoxyribonucleic acid from herring sperm is suitable for use as a blocking agent in Southern hybridizations. It was used in preparation of brain sections of mice for BrdU immunohistochemistry. It was also used for hybridization reaction of dot blot membranes.
Many factors contribute to the signal-to-noise ratio in nucleic acid hybridizations. These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane.
In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.
In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.
Notes préparatoires
This DNA is phenol-chloroform extracted, ethanol precipitated, and sonicated to produce single-stranded fragments which comigrate with the 587 and 831 base pair marker fragments.
Autres remarques
DNA in solution will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use.
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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