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P1415

Sigma-Aldrich

Prehybridization Solution

for Northern and Southern blotting, liquid (2x concentrate)

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About This Item

Code UNSPSC :
12161700
Nomenclature NACRES :
NA.25

Niveau de qualité

100
200

Forme

liquid (2x concentrate)

Activité étrangère

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

Température de stockage

−20°C

Description générale

Sigma′s Prehybridization Solution is a 2x ready-to-use buffer for Northern and Southern blot protocols.

Application

Suitable for use as a pre-hybridization buffer in Northern and Southern blot protocols.

Composants

Sigma′s 2X Prehybridization Solution contains 200 μg/mL DNA in 10x SSC and 2X Denhardt′s solutions:
  • 200 μg/mL DNA
  • 2mg Bovine Serum Albumin (BSA)
  • 2mg Ficoll
  • 2mg Polyvinylpyrrolidone (PVP)
  • 0.15 M sodium citrate
  • 1.5M NaCl

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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T M Lau et al.
Human reproduction (Oxford, England), 11(12), 2629-2634 (1996-12-01)
The expression of endometrial progesterone receptor mRNA during the human menstrual cycle and in Norplant users was studied using digoxigenin-labelled ribonucleic probes for in-situ hybridization on 6 microns paraffin embedded endometrial sections. The staining intensity was scored blind semi-quantitatively. Blood
Elizabeth S Colombo et al.
Investigative ophthalmology & visual science, 48(4), 1793-1800 (2007-03-29)
The purpose of this study was to determine the role of hepatocyte growth factor (HGF) and c-Met in the initiation and development of retinal neovascularization and to determine whether inhibition of this system can suppress the extent of angiogenesis in
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
Andrew M Pilling et al.
Toxicologic pathology, 35(3), 348-355 (2007-04-25)
Spontaneous follicular and C-cell tumors of the thyroid gland in the Han Wistar rat were examined using two morphologic procedures. Firstly, in situ hybridization (ISH) was used to localize thyroglobulin (TG) and calcitonin (CT) mRNAs. Secondly, the proteins for these
Jianmin Su et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 40(39), 7421-7435 (2020-08-28)
GABAergic interneurons represent a heterogenous group of cell types in neocortex that can be clustered based on developmental origin, morphology, physiology, and connectivity. Two abundant populations of cortical GABAergic interneurons include the low-threshold, somatostatin (SST)-expressing cells and the fast-spiking, parvalbumin

Protocoles

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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