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D3160

Sigma-Aldrich

Deoxyribonucleic acid from human placenta

buffered aqueous solution, sexed, male

Synonyme(s) :

DNA

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About This Item

Numéro CAS:
9007-49-2
Numéro MDL:
MFCD00130921
Code UNSPSC :
12352200

Qualité

for molecular biology

Forme

buffered aqueous solution

Conditions d'expédition

dry ice

Température de stockage

−20°C

InChI

1S/C15H31N3O13P2/c16-13-1-7(20)11(28-13)5-25-32(21,22)31-9-3-15(18)29-12(9)6-26-33(23,24)30-8-2-14(17)27-10(8)4-19/h7-15,19-20H,1-6,16-18H2,(H,21,22)(H,23,24)

Clé InChI

AWBASQCACWFTGD-UHFFFAOYSA-N

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Description générale

Human placental DNA is isolated from donor placenta, but will contain some maternal DNA. The DNA fragments are sonicated to produce fragments of consistent size.

Application

Deoxyribonucleic acid from human placenta is used as blocking agent in Southern hybridizations. It was used to compare the efficiency of DNA quantification methods.
For use in Southern hybridizations.

Composants

DNA is supplied in a solution of 10mM Tris-HCl (pH 8.4), with 1mM EDTA.

Produit(s) apparenté(s)

Réf. du produit
Description
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D4522

Deoxyribonucleic acid from calf thymus, Type XV, Activated, lyophilized powder

D3287

Deoxyribonucleic acid, single stranded from human placenta

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Deoxyribonucleic acid from herring sperm, single-stranded for hybridization

D7656

Deoxyribonucleic acid, single stranded from salmon testes, For hybridization

D9156

Deoxyribonucleic acid, single stranded from salmon testes, For hybridization

D3035

Deoxyribonucleic acid from human placenta, buffered aqueous solution, sexed, female

D8661

Deoxyribonucleic acid solution from calf thymus, For hybridization

S8140

SSPE Buffer, for Northern and Southern blotting, powder blend

N6019

Neutralizing Buffer, for Northern and Southern blotting, powder

N1531

Denaturation Solution, for Northern and Southern blotting

B6429

Casein Blocking Buffer 10x, for Northern and Southern blotting, solution

S2015

SSPE Buffer 20× Concentrate, for Northern and Southern blotting, solution

M5755

MOPS-EDTA-Sodium Acetate (MESA), powder for RNA electrophoresis buffers

S0902

SSC Buffer, for Northern and Southern blotting, powder blend

H7033

Tampon d′hybridation PerfectHyb™ Plus, for Northern and Southern blotting, solution

S8015

SSC Buffer, for Northern and Southern blotting, powder blend

S6639

SSC Buffer 20× Concentrate, for Northern and Southern blotting, solution

P1415

Prehybridization Solution, for Northern and Southern blotting, liquid (2x concentrate)

C7594

Casein Blocking Buffer, for Northern and Southern blotting, powder blend

15356

Blotting-Nylon 66 membranes, type B, positive, for Northern and Southern blotting

31149

Deoxyribonucleic acid, low molecular weight from salmon sperm

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Karsten Nielsen et al.
Forensic science international. Genetics, 2(3), 226-230 (2008-12-17)
Six commercial preparations of human genomic DNA were quantified using five quantification methods: UV spectrometry, SYBR-Green dye staining, slot blot hybridization with the probe D17Z1, Quantifiler Human DNA Quantification kit and RB1 rt-PCR. All methods measured higher DNA concentrations than
Maarten Holkers et al.
Nature methods, 11(10), 1051-1057 (2014-08-26)
Engineered sequence-specific nucleases and donor DNA templates can be customized to edit mammalian genomes via the homologous recombination (HR) pathway. Here we report that the nature of the donor DNA greatly affects the specificity and accuracy of the editing process
Zachary D Smith et al.
Nature, 511(7511), 611-615 (2014-08-01)
In mammals, cytosine methylation is predominantly restricted to CpG dinucleotides and stably distributed across the genome, with local, cell-type-specific regulation directed by DNA binding factors. This comparatively static landscape is in marked contrast with the events of fertilization, during which
Manolis Kellis et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(17), 6131-6138 (2014-04-23)
With the completion of the human genome sequence, attention turned to identifying and annotating its functional DNA elements. As a complement to genetic and comparative genomics approaches, the Encyclopedia of DNA Elements Project was launched to contribute maps of RNA
Hasan Yardimci et al.
Nature structural & molecular biology, 21(1), 20-25 (2014-01-07)
In G1, two copies of the MCM2-7 helicase are recruited to each origin of replication. Whereas recruitment of the first MCM2-7 is likely to be analogous to the loading of sliding clamps around DNA, how the second MCM2-7 complex is
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