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Key Documents

D8899

Sigma-Aldrich

Deoxyribonucleic acid, single stranded from calf thymus

lyophilized powder

Synonyme(s) :

DNA single stranded

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About This Item

Numéro CAS:
Numéro CE :
Numéro MDL:
Code UNSPSC :
41106310
eCl@ss :
32160414
Nomenclature NACRES :
NA.52

Qualité

for molecular biology

Niveau de qualité

Forme

lyophilized powder

Poids mol.

~50 kb

Composition

ssDNA, ≥65%

Température de stockage

−20°C

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Description générale

High quality single-stranded template DNA isolated from the thymus of male and female calves.

Application

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay and in DNA-binding assay.
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.

Caractéristiques et avantages

• Activated
• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

Quantité

One mg DNA is approx. 25 A260 units.

Notes préparatoires

Prepared by a modification of the method of Alberts and Herrick from calf thymus DNA.

Reconstitution

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves


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H Kamei
Cell biology international, 20(11), 769-775 (1996-11-01)
The anti-desmin monoclonal antibodies (MAbs) DSB389, AC54, and DSB860 recognize intermediate filaments (IFs) and nuclear antigens that appear granular, locate around chromosomes, and are insoluble following 0.5% Triton X-100 and cetyltrimethylammonium bromide (CTAB) extraction. Nuclear antigens of the MAbs were
J A Hansen et al.
Journal of animal science, 75(7), 1810-1821 (1997-07-01)
We evaluated the effects of recombinant porcine somatotropin (pST) and the beta-adrenergic agonist salbutamol on plasam endocrine and metabolite profiles and muscle chemistry in three genotypes of growing barrows (n = 96, 139 d old). Treatments were in a 2
Qiong Guo et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 106, 155-162 (2013-02-05)
A divanadium(V) complex, [V2O3(o-van-val)2] (o-van-val=Schiff base derived from o-vanillin and L-valine), has been synthesized and structurally characterized. The crystal structure shows that both of the vanadium centers in the complex have a distorted octahedral coordination environment composed of tridentate Schiff
Eswaran Ramachandran et al.
Inorganic chemistry, 52(3), 1504-1514 (2013-01-18)
A series of four new palladium(II) complexes of 2-oxo-1,2-dihydroquinoline-3-carbaldehyde thiosemicarbazones with triphenylphosphine as coligand have been synthesized and characterized by the aid of various spectral techniques. The single-crystal X-ray diffraction studies revealed that the unsubstituted thiosemicarbazone ligand acted as monobasic
Inessa Halets et al.
International journal of pharmaceutics, 454(1), 1-3 (2013-07-09)
In most articles, cytotoxicity of cationic polyamidoamine (PAMAM) dendrimers toward red blood cells has been exclusively explained by their surface charge. We have focused on dendrimer hydrophobicity as a second possible factor that determines this cytotoxicity. Using PAMAM-NH2 dendrimers from

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