Product A9594, Monoclonal ANTI-FLAG® M2-Cy3™ antibody produced in mice, is mouse IgG1. Theoretically, it will be recognized by the anti-mouse IgG secondary antibody. However, Product A9594 is already conjugated with Cy3 so a secondary antibody is not needed for detection.
A9594
Anticorps monoclonal ANTI-FLAG® M2 antibody produced in mouse
clone M2, purified immunoglobulin, buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)
Synonyme(s) :
Anti-ddddk, Anti-dykddddk
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0.2 MG
621.00 CHF
1 MG
1 500.00 CHF
5 X 1 MG
2 140.00 CHF
621.00 CHF
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Sélectionner une taille de conditionnement
Changer de vue
0.2 MG
621.00 CHF
1 MG
1 500.00 CHF
5 X 1 MG
2 140.00 CHF
About This Item
Code UNSPSC :
41106514
Nomenclature NACRES :
NA.32
621.00 CHF
Check Cart for Availability
Produits recommandés
Source biologique
mouse
Conjugué
CY3 conjugate
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
M2, monoclonal
Forme
buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)
Espèces réactives
all
Concentration
~1 mg/mL
Technique(s)
direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone
Isotype
IgG1
Séquence immunogène
DYKDDDDK
Conditions d'expédition
dry ice
Température de stockage
−20°C
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Catégories apparentées
Description générale
Monoclonal ANTI-FLAG M2-Cy3 (mouse IgG) antibody is covalently conjugated to cyanine dye Cy3. The antibody conjugate binds to FLAG fusion proteins, and will recognize the FLAG sequence at the N-terminus, Met-N-terminus, or C-terminus of FLAG fusion proteins.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
For simple, one-step detection by immunocytochemistry. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies might cause cross-reactivity.
Learn more product details in our FLAG® application portal.
Learn more product details in our FLAG® application portal.
Forme physique
Solution in phosphate buffered saline plus 1% BSA and preservative
Notes préparatoires
Dilute the antibody in Tris buffered saline (TBS): 0.05 M Tris,pH 7.4, with 0.15 M NaCl.
Autres remarques
Suggested concentration of 1-10 mg/ml for immunocytochemistry.
Informations légales
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Cy3 is a trademark of Cytiva
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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Code de la classe de stockage
10 - Combustible liquids
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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A9594（FLAG M2 Cy3標識）の抗体は抗マウスIgGの2次抗体に認識されますか。
1 answer-
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