HPLC Analysis of Protein Standards on Zenix® SEC-300 using Different Buffer Systems
Materialien
Analytische Säule
Produkt-Nr.
Beschreibung
Preisangaben
Gelfiltrations-Säule Zenix® SEC-300, 3 μm
L × I.D. 30 cm × 4.6 mm, 3 μm particle size, stainless steelKomponente der mobilen Phase
Produkt-Nr.
Beschreibung
Preisangaben
Standard
Produkt-Nr.
Beschreibung
Preisangaben
IgG aus Kaninchenserum
reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powderCONDITIONS
column
Zenix SEC-300, 30 cm x 4.6 mm I.D., 3 μm particles (Z777028)
mobile phase
[A] 0.15 M potassium phosphate monobasic, pH 7.0 with phosphoric acid; [B] 0.1 M ammonium bicarbonate (pH unadjusted)
flow rate
0.3 mL/min
column temp.
25 °C
detector
UV, 280 nm
detector
1000 psi
injection
2.5 μL
sample
in mobile phase
Beschreibung
Hinweis zur Analyse
This application compares selectivity of proteins when chromotographed by size exclusion with different buffer systems.
Rechtliche Hinweise
Zenix is a registered trademark of Sepax Technologies