Direkt zum Inhalt
Merck
  • Effect of a negative energy balance induced by feed restriction in lactating sows on hepatic lipid metabolism, milk production and development of litters.

Effect of a negative energy balance induced by feed restriction in lactating sows on hepatic lipid metabolism, milk production and development of litters.

Archives of animal nutrition (2015-08-26)
Denise K Gessner, Birthe Gröne, Susann Rosenbaum, Erika Most, Sonja Hillen, Sabrina Becker, Georg Erhardt, Gerald Reiner, Robert Ringseis, Klaus Eder
ZUSAMMENFASSUNG

In rodents, forced activation of hepatic peroxisome proliferator-activated receptor α (PPARα) by administration of exogenous PPARα activators during lactation leads to a reduction of milk triacylglycerol (TAG) production. Herein, we investigated whether a negative energy balance (NEB) induced by feed restriction (about 18% lower feed and energy intake) during lactation by increasing the release of fatty acids, which act as PPARα agonists, causes a disruption of hepatic lipid metabolism and thereby impairs milk TAG production in sows. Nutrient and energy content of the milk on day 20 of lactation and gains of litters during the first 14 d and the whole 21 d suckling period did not differ between Control and feed-restricted sows. The mRNA concentrations of several sterol regulatory element-binding protein target genes involved in lipid synthesis in the liver and the plasma concentration of TAG were reduced in the feed-restricted sows, whereas the mRNA concentrations of PPARα target genes involved in fatty acid oxidation in liver and skeletal muscle were not different between groups. In conclusion, it was shown that an NEB during lactation does not adversely affect milk composition and gains of litters, despite inhibiting hepatic expression of genes involved in lipid synthesis and reducing plasma TAG concentration. The finding that PPARα target genes involved in fatty acid utilisation in liver and muscle of sows are not induced by the NEB during lactation may explain that fatty acid availability in the mammary gland is sufficient to maintain milk TAG production and to allow normal litter gain.

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
Cholesterin, Sigma Grade, ≥99%
Sigma-Aldrich
Salzsäure -Lösung, 1.0 N, BioReagent, suitable for cell culture
Sigma-Aldrich
Salzsäure, 36.5-38.0%, BioReagent, for molecular biology
Sigma-Aldrich
Cholesterin, powder, BioReagent, suitable for cell culture, ≥99%
Supelco
Salzsäure -Lösung, volumetric, 0.1 M HCl (0.1N), endotoxin free
Sigma-Aldrich
L-Lysin -monohydrochlorid, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%
Sigma-Aldrich
Cholesterin, from sheep wool, ≥92.5% (GC), powder
Sigma-Aldrich
Chlorwasserstoff, ReagentPlus®, ≥99%
Sigma-Aldrich
Salzsäure -Lösung, ~6 M in H2O, for amino acid analysis
Sigma-Aldrich
SyntheChol® NS0-Supplement, 500 ×, synthetic cholesterol, animal component-free, aqueous solution, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Chlorwasserstoff -Lösung, 3 M in cyclopentyl methyl ether (CPME)
SAFC
Pflanzliches Cholesterin, SyntheChol®
Sigma-Aldrich
Salzsäure -Lösung, 32 wt. % in H2O, FCC
Sigma-Aldrich
L-Lysin -monohydrochlorid, reagent grade, ≥98% (HPLC)
Sigma-Aldrich
Cholesterin, from lanolin, ≥99.0% (GC)
Sigma-Aldrich
L-Lysin -monohydrochlorid, BioUltra, ≥99.5% (AT)
SAFC
Cholesterin, from sheep wool, Controlled origin, meets USP/NF testing specifications
Sigma-Aldrich
L-Carnitin-(methyl-d3) -hydrochlorid, 98 atom % D, 98% (CP)