Direkt zum Inhalt
Merck
  • Identification of a functional antioxidant responsive element in the promoter of the Chinese hamster carbonyl reductase 3 (Chcr3) gene.

Identification of a functional antioxidant responsive element in the promoter of the Chinese hamster carbonyl reductase 3 (Chcr3) gene.

Cell biology international (2015-02-14)
Takeshi Miura, Ayako Taketomi, Toshikatsu Nakabayashi, Toru Nishinaka, Tomoyuki Terada
ZUSAMMENFASSUNG

CHCR3, a member of the short-chain dehydrogenase/reductase superfamily, is a carbonyl reductase 3 enzyme in Chinese hamsters. Carbonyl reductase 3 in humans has been believed to involve the metabolism and/or pharmacokinetics of anthracycline drugs, and the mechanism underlying the gene regulation has been investigated. In this study, the nucleotide sequence of the Chcr3 promoter was originally determined, and its promoter activity was characterised. The proximal promoter region is TATA-less and GC-rich, similar to the promoter region of human carbonyl reductase 3. Cobalt stimulated the transcriptional activity of the Chcr3 gene. The results of a luciferase gene reporter assay demonstrated that cobalt-induced stimulation required an antioxidant responsive element. Forced expression of Nrf2, the transcription factor that binds to antioxidant responsive elements, enhanced the transcriptional activity of the Chcr3 gene. These results suggest that cobalt induces the expression of the Chcr3 gene via the Nrf2-antioxidant responsive element pathway.

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
Natriumchlorid, for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)
Sigma-Aldrich
Kaliumphosphat, powder, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%
Sigma-Aldrich
DL-Dithiothreitol -Lösung, BioUltra, for molecular biology, ~1 M in H2O
Supelco
DL-Dithiothreitol -Lösung, 1 M in H2O
Sigma-Aldrich
Ham's F-12 Nährstoffmischung, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Natriumchlorid -Lösung, 0.9% in water, BioXtra, suitable for cell culture
Sigma-Aldrich
Natriumchlorid, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
Sigma-Aldrich
Natriumchlorid -Lösung, 5 M in H2O, BioReagent, for molecular biology, suitable for cell culture
SAFC
Natriumchlorid -Lösung, 5 M
Sigma-Aldrich
Kaliumphosphat, for molecular biology, ≥98.0%
Sigma-Aldrich
Natriumchlorid -Lösung, BioUltra, for molecular biology, ~5 M in H2O
Sigma-Aldrich
Natriumchlorid, BioUltra, for molecular biology, ≥99.5% (AT)
Sigma-Aldrich
Kaliumphosphat, BioUltra, for molecular biology, anhydrous, ≥99.5% (T)
Sigma-Aldrich
Natriumchlorid, BioXtra, ≥99.5% (AT)
Sigma-Aldrich
Kaliumphosphat, 99.99% trace metals basis
Sigma-Aldrich
Kaliumphosphat, ReagentPlus®
Sigma-Aldrich
Natriumchlorid-35Cl, 99 atom % 35Cl
Sigma-Aldrich
Natriumchlorid -Lösung, 0.85%