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Merck

808002

TOYOPEARL® AF-Amino-650M Bulk Media

phase amine-activated, bottle of 25 mL, 65 μm particle size

Synonym(e):

TOYOPEARL® AF-Amino-650M Bulk Media

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About This Item

UNSPSC-Code:
41115700

Beschreibung

AF-Amino-650M

Produktlinie

TOYOPEARL®

Form

liquid

Verpackung

bottle of 25 mL

Hersteller/Markenname

Tosoh TOYOPEARL® AF, 08002

Kennzeichnungsgrad

100 μmol/mL±30 ligand density

Parameter

3 bar max. pressure

Methode(n)

HPLC: suitable

Matrix

hydroxylated methacrylic polymer

Aktive Matrixgruppe

amine-activated phase

Partikelgröße

65 μm

Betriebs-pH-Wert

2-13

Trenntechnik

anion exchange

Lagertemp.

2-8°C

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Allgemeine Beschreibung

Toyopearl AF-Amino-650 resin is a reactive resin used for the coupling of specific ligands for affinity chromatography. Ligands are immobilized by either peptide bond formation or reductive amination through their respective carboxylate or aldehyde groups.

Anwendung

TOYOPEARL® Affinity Media are used in affinity chromatography, protein chromatography and activated/functionalized matrices.

Physikalische Form

Shipped in 20% (v/v) ethanol.

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Rechtliche Hinweise

Toyopearl is a registered trademark of Tosoh Corporation

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Warning

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Gefahreneinstufungen

Flam. Liq. 3

WGK

WGK 1

Persönliche Schutzausrüstung

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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S Knör et al.
Journal of thrombosis and haemostasis : JTH, 6(3), 470-477 (2008-01-16)
Hemophilia A is currently treated by infusions of the coagulation factor (F) VIII, of which production and purification remain a challenging task. Current purification procedures using immunoaffinity chromatography are cumbersome, expensive, and suffer from the instability of the applied antibody
S Yamauchi et al.
The Journal of biological chemistry, 274(4), 2456-2463 (1999-01-16)
Chondroitin 4-sulfotransferase, which transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate to position 4 of N-acetylgalactosamine in chondroitin, was purified 1900-fold to apparent homogeneity with 6.1% yield from the serum-free culture medium of rat chondrosarcoma cells by affinity chromatography on heparin-Sepharose CL-6B, Matrex
Zhenzhen Wen et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(20), 1732-1740 (2011-05-17)
In order to improve its stability, immobilized Concanavalin A (Con A) on Toyopearl adsorbents was conjugated with monomethoxy poly(ethylene glycol) succinimidyl propionate (mPEG-SPA) with different molecular weight. A colorimetric method using ninhydrin is proposed to determine the degree of PEGylation;

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