SRP6045

Glucokinase from human liver

recombinant, expressed in E. coli, ≥77% (SDS-PAGE)

• Synonym(e): Hexokinase, Hexokinase, liver, Liver glucokinase
• CAS-Nummer: 9001-51-8
• UNSPSC-Code: 12352204
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human liver
• Rekombinant: expressed in E. coli
• Assay: ≥77% (SDS-PAGE)
• Form: liquid
• Mol-Gew.: 53.4 kDa
• Verpackung: pkg of 10 μg
• Konzentration: ~1 mg/mL
• Verunreinigungen: Endotoxin, tested
• NCBI-Hinterlegungsnummer: P35557
• UniProt-Hinterlegungsnummer: P35557
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... GCK(2645)

Beschreibung

Allgemeine Beschreibung

The gene encoding glucokinase (GCK) is alternatively spliced to generate three different forms of the enzyme; one found in the pancreas and two found in the liver. The enzyme is expressed as a monomer and possesses one glucose-binding site. The gene encoding GCK is localized on human chromosome 7. Recombinant human liver glucokinase (Accession# NP_277042)) has a C-terminal FLAG tag and has 474 amino acid residues.

Biochem./physiol. Wirkung

Glucose is phosphorylated to glucose-6-phosphate by glucokinases. The main function of glucokinase (GCK) is to regulate carbohydrate metabolism. The enzyme triggers the release of insulin in β-cells of pancreas. It can be useful for studies including enzyme kinetics, activator screening and kinase selectivity. Mutation in the gene encoding this protein is associated with a rare form of diabetes and the protein may also have a role in type 2 diabetes.

Einheitendefinition

One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30°C. Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2 mM ATP, 25 mM glucose, 100 μg/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human liver glucokinase at 30°C for 30 min.

Physikalische Form

50 mM potassium phosphate pH 7.4, 50 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, and 2.5% glycerol.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable