P8874

Monoclonal Anti-Phosphocan in Maus hergestellte Antikörper

~2 mg/mL, clone 122.2, purified immunoglobulin, buffered aqueous solution

• Synonym(e): Anti-PTPRB, Anti-Receptor-type Protein-tyrosine phosphatase β
• MDL-Nummer: MFCD09265428
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Konjugat: unconjugated
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 122.2, monoclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~180 kDa (higher band may be present)
• Speziesreaktivität: rat
• Verpackung: antibody small pack of 25 μL
• Konzentration: ~2 mg/mL
• Methode(n): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: 0.2-0.4 μg/mL using total extract of rat brain
• Isotyp: IgM
• UniProt-Hinterlegungsnummer: Q62656
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: rat ... Ptprz1(25613)

Beschreibung

Allgemeine Beschreibung

Monoclonal Anti-Phosphacan (mouse IgM isotype) is derived from the hybridoma 122.2 produced by the fusion of mouse myeloma cells (P3X cells) and splenocytes from BALB/c mice immunized with rat brain proteoglycans. Chondroitin sulfate proteoglycans are neural cell adhesion molecules (NCAM) ligands present in the brain extracellular matrix (ECM). Phosphacan protein is expressed mainly in astrocytes and is a ligand for NCAM. Phosphacan is the soluble extracellular domain of the receptor-type transmembrane protein tyrosine phosphatase (RPTPb).

Immunogen

rat brain proteoglycans.

Anwendung

Monoclonal Anti-Phosphacan antibody produced in mouse has been used in:

• immunoblotting
• immunohistochemistry
• immunocytochemistry.

Biochem./physiol. Wirkung

Phosphacan levels elevates during late embryogenesis. It reaches a plateau two weeks postnatal before reaching stable. Receptor-type transmembrane protein tyrosine phosphatase (RPTPb) functions to promote primary tecal neurons neurite growth, neural migration and also induces cell adhesion. Both phosphacan and RPTPb can bind to NCAM and tenascin-C and −R. Phosphacan can oppose RPTPb by competing for its binding sites. Both in hippocampal and spinal cord neurons, phosphacan can affect neuronal adhesion and neurite outgrowth.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)