The protocol is included in the product datasheet for this item. Please see the link below to review this document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/270/922/mak196bul.pdf
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MAK196
Malate Dehydrogenase Assay Kit
Sufficient for 100 Colorimetric tests
Synonym(e):
MDH Activity Assay Kit
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1 KIT
CHF 561.00
About This Item
UNSPSC-Code:
12161503
NACRES:
NA.84
Empfohlene Produkte
Nachweisverfahren
colorimetric
Relevante Krankheit(en)
neurological disorders
Lagertemp.
−20°C
Angaben zum Gen
human ... MDH1(4190) , MDH1B(130752) , MDH2(4191)
mouse ... MDH1(17449) , MDH1B(76668) , MDH2(17448)
rat ... MDH1(24551) , MDH1B(316444) , MDH2(81829)
Allgemeine Beschreibung
Malate dehydrogenase (MDH; EC 1.1.1.37) is an NAD oxidoreductase that catalyzes the NAD/NADH-dependent interconversion of malate and oxalacetate. This reaction is critical to maintain the malate/aspartate shuttle across the mitochondrial membrane and Krebs tricarboxylic acid cycle in the mitochondrial matrix. Increased MDH activity has been observed in neurodegenerative diseases such as Alzheimer′s disease.
Anwendung
Eignung
Suitable for the detection of Malate Dehydrogenase activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.
Prinzip
The Malate Dehydrogenase Assay Kit provides a simple and sensitive procedure for measuring MDH activity in a variety of tissues, cell cultures, and isolated mitochondria. MDH activity is determined by generating a product with absorbance at 450 nm proportional to the enzymatic activity present. One unit of MDH is the amount of enzyme that generates 1.0 μmole of NADH per minute at 37 °C and pH 9.5.
Nur Kit-Komponenten
Produkt-Nr.
Beschreibung
- MDH Assay Buffer
- MDH Substrate
- MDH Enzyme Mix
- MDH Developer
- NADH Standard
- MDH Positive Control
Ersetzt durch
Produkt-Nr.
Beschreibung
Preisangaben
Signalwort
Danger
H-Sätze
Gefahreneinstufungen
Eye Dam. 1 - Skin Corr. 1B
Lagerklassenschlüssel
8A - Combustible corrosive hazardous materials
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Protokolle
Spectrophotometric assay evaluates malic dehydrogenase activity using bovine heart enzyme with critical histidine residue at active site.
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Is there a protocol associated with the kit? This general protocol doesn't use MDH developer: https://www.sigmaaldrich.com/US/en/technical-documents/protocol/protein-biology/enzyme-activity-assays/_enzymatic-assay-of-malic-dehydrogenase
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