H9034

Hepatitis C virus core antigen

~1 mg/mL, ≥95% (SDS-PAGE), recombinant, expressed in E. coli

• MDL-Nummer: MFCD02264926
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Rekombinant: expressed in E. coli
• Qualitätsniveau: 200
• Assay: ≥95% (SDS-PAGE)
• Mol-Gew.: 22 kDa (plus 114 kDa galactosidse)
• Konzentration: ~1 mg/mL
• UniProt-Hinterlegungsnummer: G9G988; L7S4V3
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Angaben zum Gen: hepatitis C virus ... HCVgp1(951475) ; human ... HBVgp4(944568)

Beschreibung

Allgemeine Beschreibung

Hepatitis C virus core antigen (HCV-cAg) is a well-preserved antigenic protein of the internal capsid.

Hepatitis C virus glycoprotein 1 (HCVgp1), also referred to as polyprotein, is crucial for the induction of double-membrane vesicles, sites of HCV RNA amplification. The amino terminal of polyprotein contains the structural proteins core, envelope protein 1 (E1) and E2. The proteolytic cleavage of polyprotein results in the formation of 10 mature viral proteins which are needed for viral replication and assembly.

amino acids 2-192 of HCV polyprotein

Spezifität

Reacts strongly with human HCV positive serum.

Anwendung

Hepatitis core antigen (HCA) has been used as an analyte in enzyme-linked immunosorbent assay (ELISA) to study the mechanism of bovine serum albumin (BSA) blocking in common ELISA protocols.

Biochem./physiol. Wirkung

Hepatitis C virus core antigen (HCV-cAg) may be a less expensive way to diagnose active hepatitis C virus (HCV) infection in the hemodialysis (HD) population.

Positive control for HCV antibodies.

Sonstige Hinweise

HCV core antigen [2-192]-Galactosidase-tagged at the N-terminus.

Physikalische Form

Solution in 8 M Urea, 20 mM Tris-HCl, pH 8.0, 10 mM 2-mercaptoethanol.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable