GE28-9909-45

Superdex^® 200 Increase 5/150 GL

Cytiva 28-9909-45, column L × I.D. 15 cm × 5 mm, 8.6 μm particle size

• Synonym(e): Säule für die Größenausschlusschromatographie
• UNSPSC-Code: 41115700
• NACRES: SB.52

Eigenschaften

• Materialien: glass column; polyethylene filter
• Produktlinie: Superdex^®
• Mol-Gew.: 10000-600000 Da (globular proteins)
• Hersteller/Markenname: Cytiva 28-9909-45
• Verpackung: 1 ea of
• Parameter: <0.75 mL/min flow rate; <0.75 ml/min flow rate; <3.0 Mpa (725 psi); 4-50 μL sample volume
• Bettvolumen: ~3 mL
• Säule L × ID: 15 cm × 5 mm
• Matrix: agarose dextran composite; polymeric
• Aktive Matrixgruppe: phenyl phase
• Partikelgröße: 8.6 μm
• cleaning: 1-14
• Arbeitsbereich: 3-12
• Trenntechnik: size exclusion (SEC)

Beschreibung

Allgemeine Beschreibung

Superdex™ 200 Increase size exclusion chromatography columns are well suited for high-resolution analysis and small-scale purification of monoclonal antibodies (mAb) and other biomolecules with Mr~ 10 000 to ~ 600 000.

Superdex™ 200 Increase is a next-generation, dextran-agarose composite matrix for SEC. With smaller and more rigid beads than their predecessors, Superdex™ Increase columns deliver higher resolution purification in shorter run times.

Superdex™ 200 Increase has a selectivity curve optimized for excellent resolution of antibodies and macromolecules within the range Mr ~ 100 000 to ~ 300 000. It is well suited for detection and separation of antibody monomers, dimers, and aggregates present in monoclonal antibody preparations. The outstanding resolving power of this resin facilitates protein characterization, including analysis of antibody size variants, assessment of membrane protein size homogeneity, and the study of protein-protein interactions.

Superdex™ 200 Increase is optimized to give high resolution separation for proteins with molecular weights in the range Mr ~ 100 000 to ~ 300 000. Superose™ 6 Increase provides a complementary fractionation range, resolving very large protein complexes and macromolecules that Superdex 200 Increase cannot separate.

This agarose-based resin is alkali-resistant and supports cleaning-in-place (CIP) procedures. This capability allows the same column to be used for different proteins, with minimal risk of carry-over between samples.

Leistungsmerkmale und Vorteile

• Enhanced performance: improved resolution and runtime compared to predecessor Superdex™ 200.
• Very high resolution: small bead size and narrow particle size distribution provide high resolution, for high protein purity.
• High flow rates: rigid beads give excellent pressure/flow properties.

Eignung

Gut geeignet für Reinheits-Schnelltests und rasche Screenings von experimentellen Bedingungen.

Langfristige Verwendung: alle gebräuchlichen wässrigen Puffer, bis zu 8 M Harnstoff, ionische und nicht-ionische Reinigungsmittel, bis zu 6 M Guanidinhydrochlorid

Lagerung und Haltbarkeit

Bei 4 bis 30 °C aufbewahren (20 % Ethanol)

Bitte beachten Sie, dass dieses Produkt möglicherweise 90 Tage vor dem Verfallsdatum versandt wird. Für weitere Informationen zum chargenspezifischen Verfallsdatum wenden Sie sich bitte an den technischen Kundendienst.

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Rechtliche Hinweise

Superdex is a registered trademark of Cytiva

Sicherheitshinweise

• Piktogramme: GHS02
• Signalwort: Warning
• H-Sätze: H226
• P-Sätze: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Lagerklassenschlüssel: 3 - Flammable liquids