GE17-5130-01

GSTrap^™ Fast Flow

Cytiva 17-5130-01, pack of 5 × 1 mL

• Synonym(e): GSTrap column
• UNSPSC-Code: 23151817
• NACRES: NA.25

Eigenschaften

• ligand: glutathione and 10-carbon linker arm
• Verpackung: pack of 5 × 1 mL
• Hersteller/Markenname: Cytiva 17-5130-01
• Parameter: flow rate; 42 psi
• Bettgröße: 7 mm × 25 mm
• Bettvolumen: 1 mL
• Säule ID: 7 mm
• Matrix: highly cross-linked 4% agarose
• Durchschnittlicher Durchmesser: 90 μm
• cleaning: 3-12
• working range: 3-12
• Kapazität: >10 mg/mL binding capacity

Beschreibung

Allgemeine Beschreibung

GSTrap^™ FF columns are prepacked Glutathione Sepharose^™ Fast Flow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins.

GST*-tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap^™ FF.GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose^™ 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as AKTA design or FPLC^™ System.

* See the section for licensing information in our terms and conditions.

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Leistungsmerkmale und Vorteile

• Fast and simple one-step purification of GST-tagged proteins.
• Mild elution conditions preserving protein antigenicity and function.
• Easy scale-up by connecting the columns in series.
• Sepharose^™ 4 Fast Flow provides good flow properties.

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Rechtliche Hinweise

FPLC is a trademark of Cytiva

GSTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

Sicherheitshinweise

• Piktogramme: GHS02
• Signalwort: Warning
• H-Sätze: H226
• P-Sätze: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Lagerklassenschlüssel: 3 - Flammable liquids