This will fluctuate due to sample variability (sample collection, concentration, fragment length, sequence [GC content], storage before isolation, etc.). However, the sample is buffer exchanged into a standard storage buffer that is included in the kit (1X TE).
EC196
GenElute™-E Single Spin Blood DNA 96 Kit
Reagents and consumables for various blood sample types. 96-well plate format.
Synonym(e):
GenElute™-E Blood DNA 96 Kit, GenElute™-E Single Spin Kit For DNA purification, GenElute™-E purification kit
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NACRES:
NA.52
UNSPSC Code:
41105600
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Unterstützung erhaltenpurified by
(Single-spin negative chromotography)
feature
Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS), Intended use (For the purification of genomic DNA from whole or dried blood samples), Typical/expected yield (Varies by sample. Please reference user guide for more information.)
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
DNA purification: suitable
input
blood (liquid or dried)
test parameters
: 3 min hands on time, sample volume: 60 μL blood or 2-5 dried blood spots
greener alternative category
, Aligned
storage temp.
2-8°C
1 of 4
Dieser Artikel | |||
|---|---|---|---|
| technique(s) DNA purification: suitable | technique(s) - | technique(s) - | technique(s) - |
| purified by (Single-spin negative chromotography) | purified by (Single-spin negative chromotography), (Time: 45 minutes or less) | purified by (Single-spin negative chromotography), (Time: 30 minutes or less) | purified by (Single-spin negative chromotography) |
| feature Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS) | feature Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS), Intended use (For the purification of genomic DNA from whole or dried blood samples), Typical/expected yield (Varies by sample. Please reference user guide for more information.) | feature Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS), Intended use (For the purification of genomic DNA from liquid blood samples), Typical/expected yield (Varies by sample. Please reference user guide for more information.) | feature Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS), Intended use (For the purificiation of genomic DNA from human or animal tissue), Typical/expected yield (Varies by sample. Please reference user guide for more information.) |
| greener alternative product characteristics Waste Prevention | greener alternative product characteristics Waste Prevention | greener alternative product characteristics Waste Prevention | greener alternative product characteristics Waste Prevention |
| sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product |
| greener alternative category , Aligned | greener alternative category , Aligned | greener alternative category | greener alternative category , Aligned |
General description
GenElute™-E purification kits provide a simple alternative for DNA purification. Compared with traditional methods, GenElute™-E offers:
Traditional silica-based, bind-wash-elute purification kits require multiple wash steps to remove impurities from the spin columns. These steps increase the risk of cross contamination, subject the DNA to centrifugation sheering forces, and introduce chaotropic salts that can carry over into the final sample, inhibiting downstream applications.
GenElute™-E kits employ size exclusion negative chromatography to separate large nucleic acid molecules from smaller protein, lipid, and ionic components in cell, tissue, blood, and other samples. Single-spin columns efficiently absorb and retain cellular debris and sample contaminants while allowing nucleic acids to pass through, reducing the number of steps and plastic materials required for purification. This novel method for high-quality purification is made possible by our innovative SmartLyse® Protease, which enables fast and efficient lysis of a wide range of sample types.
Isolate nucleic acid in a fraction of time compared to traditional silica bind-wash-elute procedures. Simply mix sample with lysis components and incubate according to kit protocol, pipette sample onto the spin column, and centrifuge directly into a collection tube. Cellular debris and contaminants remain bound in the column to be discarded, while purified DNA is ready to be used in downstream applications or stored.
- Isolation of DNA in under an hour with minimal sample handling
- Better purity, leading to improved performance in PCR and other applications
- Significant reduction in plastic and hazardous chemical waste
Traditional silica-based, bind-wash-elute purification kits require multiple wash steps to remove impurities from the spin columns. These steps increase the risk of cross contamination, subject the DNA to centrifugation sheering forces, and introduce chaotropic salts that can carry over into the final sample, inhibiting downstream applications.
GenElute™-E kits employ size exclusion negative chromatography to separate large nucleic acid molecules from smaller protein, lipid, and ionic components in cell, tissue, blood, and other samples. Single-spin columns efficiently absorb and retain cellular debris and sample contaminants while allowing nucleic acids to pass through, reducing the number of steps and plastic materials required for purification. This novel method for high-quality purification is made possible by our innovative SmartLyse® Protease, which enables fast and efficient lysis of a wide range of sample types.
Isolate nucleic acid in a fraction of time compared to traditional silica bind-wash-elute procedures. Simply mix sample with lysis components and incubate according to kit protocol, pipette sample onto the spin column, and centrifuge directly into a collection tube. Cellular debris and contaminants remain bound in the column to be discarded, while purified DNA is ready to be used in downstream applications or stored.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is aligned with "Waste prevention" and "Designing safer chemicals". Click here for more information.
Application
The GenElute™-E Single Spin Blood DNA 96 Kit has been developed to purify genomic DNA from a wide variety of blood samples, including fresh, frozen or whole blood or dried blood spots (FTA card punches). EDTA-, citrate- as well as heparin-stabilized human and animal blood can be used.
The initial blood lysis step takes place under physiological conditions where the enzyme activity of proteases in general is increased. SmartLyse® Protease contains a unique combination of several active enzymes with an expanded substrate specificity. This optimization allows for fast and efficient lysis of blood cells (from, e. g., human, animal, fish, and birds) and dried blood spots (coated FTA cards) with the same protocol.
Purified genomic DNA is eluted in Tris buffer, pH 7.8 and can immediately be used for most common downstream applications, including PCR, genotyping, NGS, and others. Final yield is comparable to most common silica-based methods. GenElute™-E purified genomic DNA preparations commonly show an A260/280 ratio of around 1.8.
The initial blood lysis step takes place under physiological conditions where the enzyme activity of proteases in general is increased. SmartLyse® Protease contains a unique combination of several active enzymes with an expanded substrate specificity. This optimization allows for fast and efficient lysis of blood cells (from, e. g., human, animal, fish, and birds) and dried blood spots (coated FTA cards) with the same protocol.
Purified genomic DNA is eluted in Tris buffer, pH 7.8 and can immediately be used for most common downstream applications, including PCR, genotyping, NGS, and others. Final yield is comparable to most common silica-based methods. GenElute™-E purified genomic DNA preparations commonly show an A260/280 ratio of around 1.8.
Features and Benefits
Fast and efficient purification: 40-minute lysis and minimal hands-on, preparation stepsHigher quality DNA: negative chromotography-based purification reduces carryover of PCR inhibitors and other contaminents Reduced environmental impact: 55% less plastic waste, as well as a significant reduction in hazardous chemicals
Preparation Note
Store enzymes and purification plates at 2-8°C for up to 12 months. All other components should be stored at room temperature.
Other Notes
We believe that green chemistry will contribute to a better tomorrow. With a growing portfolio of greener alternatives, there are now more choices to reduce the ecological impact of your research while still delivering quality and efficacy so your results are not compromised. GenElute™-E purification kits avoid the use of hazardous wash buffers typically employed in silica-based purification kits, significantly reducing chemical waste. The single-spin protocol also limits the number of collection steps, reducing plastic waste by 55% compared to equivalent bind-wash-elute methods. GenElute™-E is designated a Greener Alternative Product based on the "Prevention" and "Designing Safer Chemicals" qualifications of The 12 Principles of Green Chemistry.GenElute™-E kits also adhere to the principles of SMASH Packaging, our global initiative to improve the sustainability through the reduction of packaging materials, the increased use of sustainable alternatives, and improved recyclability. Packaging is FCS-marked for sustainable forestry practices and produced from greater than 70% recycled content.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
SmartLyse is a registered trademark of Merck KGaA, Darmstadt, Germany
Nur Kit-Komponenten
Produkt-Nr.
Beschreibung
- Blood Lysis Buffer
- SmartLyse® B Protease
- Clearing Solution B
- 1x Tris Buffer
- Lysis Plate
- Purification Plate
- DNA Storate Plate
- Adhesive Foil
Alle anzeigen (8)
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Lagerklasse
10 - Combustible liquids
wgk
WGK 3
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Artikel
Impatto del metodo di purificazione sull'accuratezza della quantificazione del DNA e sulle procedure enzimatiche successive. Valutazione mediante spettrofotometria UV, elettroforesi su gel e successiva qPCR della purezza del DNA genomico purificato con i kit GenElute™-E.
Verwandter Inhalt
Animation showing technology principle of GenElute™-E single spin negative chromatography nucleic acid purification kits
Demo video showing how to purify DNA and RNA GenElute™-E single spin nucleic acid purification kits
Answers to frequently asked questions related to GenElute™-E single spin DNA and RNA purification and negative chromatography
Reduce plastic waste and eliminate hazardous liquid waste for more sustainable laboratories with GenElute™-E Single Spin DNA and RNA prep kits.
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Do we know how stable the purified DNA is through several freeze-thaw cycles?
1 answer-
Helpful?
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Genelute-e single spin DNA and RNA purification kits use negative chromatographty to isolate nucleic acids. Can you explain how this approach simplifies workflows?
1 answer-
Instead of optimizing the bind, wash, and release steps in conventional silica-based spin purification preps, the technology focuses on a separation by performing a single step fractionation based on the size of the biomolecules, which results in depleted impurities.
Helpful?
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Does the technology introduce any bias into the sample?
1 answer-
GenElute™-E does not introduce biases that some “bind-wash-elute” technologies can add because the technology separates by size, rather than by what binds and what is released.
Helpful?
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What is the composition of the lysis buffer and clearing buffer after flowing through the resin?
1 answer-
The presence of EDTA, SDS, or excess salt can affect my PCR/ sequencing reaction. The lysis buffer information is proprietary, but we can say it is free of chaotropic salts. The resins are desalting resins so EDTA, SDS, and salts are depleted.
Helpful?
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