EC100

GenElute^™-E Single Spin Blood DNA Kit

Reagents and consumables for various blood sample types. 10, 50 or 250 purifications.

• Synonym(e): GenElute^™-E Blood DNA Kit
• NACRES: NA.52
• UNSPSC Code: 41105600

Eigenschaften

• purified by: (Single-spin negative chromotography), (Time: 45 minutes or less)
• feature: Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS), Intended use (For the purification of genomic DNA from whole or dried blood samples), Typical/expected yield (Varies by sample. Please reference user guide for more information.)
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• technique(s): DNA purification: suitable
• input: blood (liquid or dried)
• test parameters: : 3 min hands on time, sample volume: 60 μL blood or 2-5 dried blood spots
• greener alternative category: , Aligned
• storage temp.: 2-8°C

Beschreibung

General description

Click here to view the kit protocol

GenElute^™-E purification kits provide a simple alternative for DNA purification. Compared with traditional methods, GenElute^™-E offers:

• Isolation of DNA in under an hour with minimal sample handling
• Better purity, leading to improved performance in PCR and other applications
• Significant reduction in plastic and hazardous chemical waste

Traditional silica-based, bind-wash-elute purification kits require multiple wash steps to remove impurities from the spin columns. These steps increase the risk of cross contamination, subject the DNA to centrifugation sheering forces, and introduce chaotropic salts that can carry over into the final sample, inhibiting downstream applications.

GenElute^™-E kits employ size exclusion negative chromatography to separate large nucleic acid molecules from smaller protein, lipid, and ionic components in cell, tissue, blood, and other samples. Single-spin columns efficiently absorb and retain cellular debris and sample contaminants while allowing nucleic acids to pass through, reducing the number of steps and plastic materials required for purification. This novel method for high-quality purification is made possible by our innovative SmartLyse^® Protease, which enables fast and efficient lysis of a wide range of sample types.

Isolate nucleic acid in a fraction of time compared to traditional silica bind-wash-elute procedures. Simply mix sample with lysis components and incubate according to kit protocol, pipette sample onto the spin column, and centrifuge directly into a collection tube. Cellular debris and contaminants remain bound in the column to be discarded, while purified DNA is ready to be used in downstream applications or stored.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is aligned with "Waste prevention" and "Designing safer chemicals". Click here for more information.

Application

Store enzymes at 2-8°C for up to 12 months. All other components should be stored at room temperature.

The GenElute^™-E Single Spin Blood DNA Kit has been developed to purify genomic DNA from a wide variety of blood samples, including fresh, frozen or whole blood or dried blood spots (FTA card punches). EDTA-, citrate- as well as heparin-stabilized human and animal blood can be used.

The initial blood lysis step takes place under physiological conditions where the enzyme activity of proteases in general is increased. SmartLyse^® Protease contains a unique combination of several active enzymes with an expanded substrate specificity. This optimization allows for fast and efficient lysis of blood cells (from, e. g., human, animal, fish, and birds) and dried blood spots (coated FTA cards) with the same protocol.

Purified genomic DNA is eluted in Tris buffer, pH 7.8 and can immediately be used for most common downstream applications, including PCR, genotyping, NGS, and others. Final yield is comparable to most common silica-based methods. GenElute^™-E purified genomic DNA preparations commonly show an A260/280 ratio of around 1.8.

Features and Benefits

Fast and efficient purification: 40-minute lysis and minimal hands-on, preparation stepsHigher quality DNA: negative chromotography-based purification reduces carryover of PCR inhibitors and other contaminents Reduced environmental impact: 55% less plastic waste, as well as a significant reduction in hazardous chemicals

Other Notes

We believe that green chemistry will contribute to a better tomorrow. With a growing portfolio of greener alternatives, there are now more choices to reduce the ecological impact of your research while still delivering quality and efficacy so your results are not compromised.  GenElute^™-E purification kits avoid the use of hazardous wash buffers typically employed in silica-based purification kits, significantly reducing chemical waste. The single-spin protocol also limits the number of collection steps, reducing plastic waste by 55% compared to equivalent bind-wash-elute methods. GenElute^™-E is designated a Greener Alternative Product based on the "Prevention" and "Designing Safer Chemicals" qualifications of The 12 Principles of Green Chemistry.GenElute^™-E kits also adhere to the principles of SMASH Packaging, our global initiative to improve the sustainability through the reduction of packaging materials, the increased use of sustainable alternatives, and improved recyclability. Packaging is FCS-marked for sustainable forestry practices and produced from greater than 70% recycled content.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

SmartLyse is a registered trademark of Merck KGaA, Darmstadt, Germany

Nur Kit-Komponenten

• Blood Lysis Buffer
• SmartLyse^® B Protease
• Clearing Solution B
• 1x Tris Buffer
• Spin Columns

Sicherheitshinweise

• pictograms: GHS05,GHS07,GHS08
• signalword: Danger
• hcodes: H315 - H318 - H334 - H335 - H412
• pcodes: P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 3