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D7876
Diamine Oxidase from porcine kidney
≥0.05 unit/mg solid
Synonym(e):
Amine:oxygen oxidoreductase (deaminating) (pyridoxal-containing)
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Größe auswählen
250 MG
CHF 108.00
1 G
CHF 361.00
5 G
CHF 1’300.00
10 G
CHF 2’120.00
Alle Fotos(4)
Größe auswählen
Ansicht ändern
250 MG
CHF 108.00
1 G
CHF 361.00
5 G
CHF 1’300.00
10 G
CHF 2’120.00
About This Item
Empfohlene Produkte
Biologische Quelle
Porcine kidney
Form
solid
Spezifische Aktivität
≥0.05 unit/mg solid
Mol-Gew.
170 kDa
Löslichkeit
100 mM sodium phosphate buffer, pH 7.2: soluble 10 mg/mL
Fremdaktivität
monoamine oxidase (benzylamine substrate) ≤1%
Lagertemp.
−20°C
Allgemeine Beschreibung
Diamine oxidase from porcine kidney is a homodimer consisting of two equal subunits with a molecular weight of 87 kDa each. Each subunit contains one molecule of pyridoxal phosphate and one atom of copper.[1] The molecular mass of the enzyme is found to be 170 kDa.[2] The enzyme is a glycoprotein containing 5% hexose, 3.3% glucosamine, 2.6% N-acetylglucosamine, and 0.25% N-acetylneuraminic acid. The enzyme exhibits a high affinity for concanavalin A.[3] Optimum pH with cadverine and histamine as substrates is found to be 6.3-7.4.[1]
Anwendung
Diamine Oxidase from porcine kidney has been used in the construction of histamine biosensor.[4]
Diamine oxidase from porcine kidney has been used in a study to investigate a luminescence-based test for determining ornithine decarboxylase activity. Diamine oxidase from porcine kidney has also been used in a study to investigate N-linked oligosaccharide structures in diamine oxidase.
Biochem./physiol. Wirkung
Diamine Oxidase catalyzes the oxidation of monoamines, diamines, and histamine to aldehydes, ammonia, and hydrogen peroxide.[5] The enzyme is classified as a copper amine oxidase and it is a key enzyme in nitrogen metabolism.[6] Diamine oxidase is inhibited by diethyldithiocarbamate, phenylhydrazine, semicarbazide, cyanide, isonicotinic acid hydrazide.[7]
Einheitendefinition
One unit will oxidize 1.0 μmole of putrescine per hr at pH 7.2 at 37 °C.
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
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Marta Moniente et al.
Food research international (Ottawa, Ont.), 160, 111735-111735 (2022-09-10)
Lentilactobacillus parabuchneri is the main bacteria responsible for the accumulation of histamine in cheese. The goal of this study was to assess the efficiency of potential histamine-degrading microbial strains or, alternatively, the action of the diamine oxidase (DAO) enzyme in
M A Shah et al.
The Biochemical journal, 253(1), 103-107 (1988-07-01)
Pig kidney diamine oxidase (DAO) was found to contain 5% (w/w) natural hexose, 3.25% glucosamine, 2.61% N-acetylglucosamine and 0.25% N-acetylneuraminic acid. The enzyme exhibited strong affinity towards concanavalin A (Con A) with a stoichiometry of 1:4.6. The kinetics of interaction
Y Huang et al.
Carbohydrate research, 323(1-4), 111-125 (2000-04-27)
Structures of the N-linked glycans released from porcine kidney diamine oxidase (DAO) were characterized utilizing various analytical techniques, including matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI/TOF-MS), high-performance capillary electrophoresis (HPCE), and high-pH anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). The
Diamine oxidase and catalase are expressed in the same cells but are present in different subcellular compartments in porcine kidney.
H G Schwelberger et al.
Inflammation research : official journal of the European Histamine Research Society ... [et al.], 48 Suppl 1, S81-S82 (1999-06-01)
A Rinaldi et al.
Preparative biochemistry, 12(1), 11-28 (1982-01-01)
Several methods for the isolation of apparently homogeneous pig kidney diamine oxidase have been reported in recent years (1-7), but these procedures allow to obtain only little amounts of material making very difficult the study of the molecular properties of
Protokolle
To standardize a procedure for the enzymatic assay of Diamine Oxidase.
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