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CHROP
Imprint® Ultra Chromatin Optimization Kit
Kit designed to optimize sonication parameters for ChIP experiments
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| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|---|---|---|
| 15 reactions | Warenkorb auf Verfügbarkeit prüfen | CHF 323.00 |
Über diesen Artikel
NACRES:
NA.54
UNSPSC Code:
41116158
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General description
The Imprint Ultra Chromatin Optimization Kit provides reagents needed to optimize chromatin sonication/shearing conditions before performing ChIP. It is imperative that chromatin is completely sheared into fragments with a peak around 500 base pairs (bp) for ChIP-qPCR assays, and < 500 bp for ChIP-Seq and ChIP-chip, for maximum specificity and resolution, and to ensure low background. For higher resolution applications like genome-wide location analysis by ChIP-chip or ChIP-Seq, sonication of input chromatin to below 500 bp, with a peak around 250 bp, is critical.
Other Notes
Sufficient reagents are provided to perform three sets of chromatin sonication optimization experiments. Each set would include five different conditions for sonication to optimize the parameters. Reagents are provided for a total of 15 reactions, including preparation of cell lysates from 107 cells/reaction, nuclei lysis, sonication, cross-link reversal and DNA purification.
Legal Information
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
Kit-Komponenten auch einzeln erhältlich
Produkt-Nr.
Beschreibung
SDB
- I8896IGEPAL® CA-630, Molecular Biology
- S5150Sodium chloride solution, 5 M in H2O, BioReagent, Molecular Biology
- W4502Water, Nuclease-Free Water, for Molecular Biology
- A84564-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, ≥97.0% (HPLC)
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution
- R4642Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)
signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Ox. Liq. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3
target_organs
Central nervous system
Lagerklasse
5.1A - Strongly oxidizing hazardous materials
flash_point_f
100.4 °F
flash_point_c
38 °C
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Henriette O'Geen et al.
BioTechniques, 41(5), 577-580 (2006-12-05)
A single chromatin immunoprecipitation (ChIP) sample does not provide enough DNA for hybridization to a genomic tiling array. A commonly used technique for amplifying the DNA obtained from ChIP assays is ligation-mediated PCR (LM-PCR). However; using this amplification method, we
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Proceedings of the National Academy of Sciences of the USA, 1, 13641-13646 (2011)
M H Kuo et al.
Methods (San Diego, Calif.), 19(3), 425-433 (1999-12-02)
Chromatin structure plays important roles in regulating many DNA-templated processes, such as transcription, replication, and recombination. Considerable progress has recently been made in the identification of large, multisubunit complexes dedicated to these nuclear processes, all of which occur on nucleosomal
Yoon-A Kang et al.
Molecular and cellular biology, 32(1), 226-239 (2011-10-26)
Developmental and homeostatic remodeling of cellular organelles is mediated by a complex process termed autophagy. The cohort of proteins that constitute the autophagy machinery functions in a multistep biochemical pathway. Though components of the autophagy machinery are broadly expressed, autophagy
Protokolle
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
Verwandter Inhalt
Imprint® Ultra Chromatin IP Kit: for Successful ChIP-Seq with a Low Abundance Transcription Factor
Global Trade Item Number
| SKU | GTIN |
|---|---|
| CHROP-15RXN | 04061833296080 |
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