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Imprint® Ultra Chromatin Optimization Kit
Kit designed to optimize sonication parameters for ChIP experiments
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15 REACTIONS
CHF 310.00
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15 REACTIONS
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About This Item
UNSPSC-Code:
41116158
NACRES:
NA.54
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Allgemeine Beschreibung
The Imprint Ultra Chromatin Optimization Kit provides reagents needed to optimize chromatin sonication/shearing conditions before performing ChIP. It is imperative that chromatin is completely sheared into fragments with a peak around 500 base pairs (bp) for ChIP-qPCR assays, and < 500 bp for ChIP-Seq and ChIP-chip, for maximum specificity and resolution, and to ensure low background. For higher resolution applications like genome-wide location analysis by ChIP-chip or ChIP-Seq, sonication of input chromatin to below 500 bp, with a peak around 250 bp, is critical.
Komponenten
Sufficient reagents are provided to perform three sets of chromatin sonication optimization experiments. Each set would include five different conditions for sonication to optimize the parameters. Reagents are provided for a total of 15 reactions, including preparation of cell lysates from 107 cells/reaction, nuclei lysis, sonication, cross-link reversal and DNA purification.
Rechtliche Hinweise
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
Kit-Komponenten auch einzeln erhältlich
Produkt-Nr.
Beschreibung
SDB
- I8896IGEPAL® CA-630, for molecular biologySDB
- S5150Sodium chloride solution, 5 M in H2O, BioReagent, for molecular biology, suitable for cell cultureSDB
- W4502Water, Nuclease-Free Water, for Molecular BiologySDB
- A84564-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, ≥97.0% (HPLC)SDB
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solutionSDB
- R4642Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)SDB
Signalwort
Danger
Gefahreneinstufungen
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Ox. Liq. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3
Zielorgane
Central nervous system
Lagerklassenschlüssel
5.1A - Strongly oxidizing hazardous materials
Flammpunkt (°F)
100.4 °F
Flammpunkt (°C)
38 °C
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A single chromatin immunoprecipitation (ChIP) sample does not provide enough DNA for hybridization to a genomic tiling array. A commonly used technique for amplifying the DNA obtained from ChIP assays is ligation-mediated PCR (LM-PCR). However; using this amplification method, we
Yoon-A Kang et al.
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M H Kuo et al.
Methods (San Diego, Calif.), 19(3), 425-433 (1999-12-02)
Chromatin structure plays important roles in regulating many DNA-templated processes, such as transcription, replication, and recombination. Considerable progress has recently been made in the identification of large, multisubunit complexes dedicated to these nuclear processes, all of which occur on nucleosomal
Protokolle
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
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