XrS6-hamKu80

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: hamster ovary
• Verpackung: tube of 5 μg 96012301-DNA-5UG; pkg of vial of cells 96012301-1VL
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Not specified
• Produkte: Ku80
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Hamster Chinese ovary, transformed with hamster Ku80 cDNA

Beschreibung der Zelllinie

The cell line Xrs6-hamKu80 was derived from a radiosensitive CHO-K1 mutant transfected with hamster Ku80 cDNA. Ku80, a subunit of the Ku protein and component of the DNA-dependent protein kinase, is a gene product defective in radiosensitive CHO cells, so called xrs mutants. XRC5, the gene encoding Ku80, belongs to the ionisation radiation group affected in DNA repair defects. Mutants transfected with hamster Ku80 obtained full restoration of gamma-ray resistance, DNA end-binding activity to parent levels and expression of Ku80 and Ku70 proteins.

Anwendung

Study of DNA repair mechanisms

Nährmedium

Ham′s F12 + 2mM Glutamine + 300μg/ml G418 + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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