C8740 does not interfere with downstream applications such as 6X HisTag affinity tag purification, or detection on a Western Blot with an anti-6X His antibody.
If the intention is to conjugate a protein to a 6X HisTag, the protein should first be purified from the lysate before conjugation. C8740 will not interfere with a conjugation protocol after the protein of interest has been isolated from the lysate.
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C8740
CelLytic™ B-Zelllysereagenz
For bacterial cell lysis, 10× concentrate
Synonym(e):
bacterial cell lysis reagent
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Größe auswählen
10 ML
CHF 112.00
50 ML
CHF 435.00
100 ML
CHF 595.00
CHF 112.00
Voraussichtliches Versanddatum15. April 2025
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Größe auswählen
Ansicht ändern
10 ML
CHF 112.00
50 ML
CHF 435.00
100 ML
CHF 595.00
About This Item
CHF 112.00
Voraussichtliches Versanddatum15. April 2025
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Qualitätsniveau
Form
liquid
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Anwendung
Eine proprietäre, nicht denaturierende Formulierung zwitterionischer Detergenzien, die für die Lyse von bakteriellen Zellen und die Extraktion von rekombinanten Proteinen verwendet wird.
Leistungsmerkmale und Vorteile
- Höhere Wirksamkeit bei der Proteinextraktion als herkömmliche Methoden wie Ultraschall und Lysozym.
- Skalierbar für 1–25 Gramm bakterieller Zellpaste
- Keine Interferenzen mit nachgeschalteten Anwendungen wie Affinitätschromatographie, IP und Western-Blot
- Kompatibel mit Protease-Hemmern, -Hemmer-Cocktails, chaotropen Substanzen, Salzen, Chelatbildnern und Reduktionsmitteln
Sonstige Hinweise
Das 10X-Konzentrat der Detergensmischung enthält keine Pufferkomponenten und ermöglicht den Forschenden die Flexibilität, das Lysereagens in das gewünschte System zu integrieren. Es eignet sich auch für Automatisierungsprotokolle für die Extraktion und Aufreinigung von rekombinanten Proteinen.
Rechtliche Hinweise
Unterliegen dem US-Patent Nr. 7.282.475 B2 und werden ausschließlich für Forschungszwecke verkauft. Die kommerzielle Verwendung erfordert zusätzliche Lizenzen.
CelLytic is a trademark of Sigma-Aldrich Co. LLC
Signalwort
Warning
H-Sätze
Gefahreneinstufungen
Eye Irrit. 2 - Skin Irrit. 2
Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
Hier finden Sie alle aktuellen Versionen:
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Can this product safe to be used for downstream crosslinking stains such as a 6x HIsTag?
1 answer-
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When using the CelLytic™ B lysis reagent, do I need to make a dilution prior to measuring protein by Bradford assay?
1 answer-
When using the CelLytic™ B lysis reagent (B7435, B7310, C8740, and contained in kits CB0050 and CB0500), it is best to use a 1:10 dilution in order to reduce the risk of interference with the Bradford Reagent. A 1:20 dilution would be even better, as long as the protein level remains within the standard curve.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What bacterial cells can be used with CelLytic B?
1 answer-
CelLytic B Reagent by itself (no lysozyme or benzonase) was optimized for E. coli strain BL21. It also works with other common E. coli strains such as DH5?, JM109, and HB 101. The CelLytic B Reagent may be used on other similar bacterial cells. The CelLytic B Plus Kit is compatible with many more difficult to lyse Gram positive bacteria such as Bacillus and Streptococcus.
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Can I use the CelLytic™ B cell lysate in an activity assay?
1 answer-
CelLytic B contains a zwitterionic detergent so the protein is active and can be used in protein activity assays.
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What is the difference between CelLytic B (Product Nos. B7435, B7310, C8740) and CelLytic B Plus (Product Nos. CB0050 and CB0500)?
1 answer-
CelLytic B Plus contains CelLytic B, Product No. B7435 as well as lysozyme, benzonase, and a protease inhibitor cocktail for a complete reagent for cell lysates for protein isolation.
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What is the difference between CelLytic B (Product No. B7435), CelLytic B 2x (Product No. B7310) and CelLytic B 10x (Product No. C8740)?
1 answer-
CelLytic B 2X has double the strength of CelLytic B for small volume extractions. This allows CelLytic B 2X to be used when a higher protein concentration or lower volume is required. The original formula, CelLytic B, requires 10-20 ml of the reagent for 1 gram of wet cell paste. Only 5 ml of the CelLytic B 2X reagent is required to lyse and extract protein from 1 gram of wet cell paste. CelLytic B 10X is provided as a concentrated mixture of the proprietary detergents found in CelLytic B, without any buffering component. This allows the user to customize the extraction reagent by choosing the detergent concentration and buffering components ideal for the extraction and purification of the protein(s) of interest. CelLytic B 10X is also suitable for the lysis of yeast and mammalian cells.
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Can I obtain protein from inclusion bodies with CelLytic B?
1 answer-
If the protein is found in inclusion bodies, you need to use an additional reagent, Product No. C5236, CelLytic IB to solubilize the proteins from the inclusion bodies. CelLytic B is used to isolate the inclusion bodies from the bacteria. CelLytic IB, Product No. C5236 can then be used to solubilize the proteins from the inclusion bodies.
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Can I concentrate my sample that was made using CelLytic B?
1 answer-
If concentrating a sample, be aware that concentration using filter concentrators may increase the detergent concentration significantly and cause issues in downstream applications. heoretically, ways to get around this are TCA precipitation, or diluting the extract below the CMC prior to concentration. This would be at least a 1/5 dilution, based on the following reference: Saito, S., and Tsuchiya, T., Characteristics of n-octyl beta-D-thioglucopyranoside, a new non-ionic detergent useful for membrane biochemistry. Biochem. J., 222(3), 829-832, 1984.
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Can I use CelLytic B with the maltose binding protein system (MBP System)?
1 answer-
CelLytic B cannot be used with the MBP system. We have used this in the maltose binding protein system, and know that it will NOT work in this system, since any and all detergents will destroy binding of protein to the resin.
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