C8374

Human Collagen Type IV

from human placenta, powder

• Synonym(e): Kollagen aus menschlicher Placenta
• CAS-Nummer: 9007-34-5
• EG-Nummer: 232-697-4
• MDL-Nummer: MFCD00130825
• UNSPSC-Code: 12352202
• NACRES: NA.61

Eigenschaften

• product name: Kollagen aus menschlicher Placenta, Bornstein and Traub Type IV, powder
• Biologische Quelle: human placenta
• Qualitätsniveau: 200
• Assay: >85% (SDS-PAGE)
• Form: powder
• Methode(n): cell culture | stem cell: suitable
• Verunreinigungen: HIV, hepatitis B and hepatitis C, none detected
• Löslichkeit: 0.5 M acetic acid: 1 mg/mL, clear to very slightly hazy
• UniProt-Hinterlegungsnummer: P02462; P08572; P29400; P53420; Q01955; Q14031
• Lagertemp.: −20°C
• Angaben zum Gen: human ... COL4A1(1282) , COL4A2(1284) , COL4A3(1285) , COL4A4(1286) , COL4A5(1287) , COL4A6(1288)

Beschreibung

Anwendung

Type IV collagen has been found to play a key role in angiogenesis, neurological diseases and metastasis. Collagen from human placenta has been used to assess the bioelectric effects of quinine on airway epithelial cells, to study the selective toxicity of engineered lentvirus lytic peptides and in a particle aggregation assay for the rapid detection of fibronectin, fibrinogen and collagen receptors on Staphylococcus aureus. It has also been used in a magnesium-dependent, collagen-binding assay during characterization of human lung tumor-associated antigens.

Biochem./physiol. Wirkung

During development, collagen IV is ubiquitously distributed in BMs. During the maturation process, this network gets partially replaced in a remarkably tissue specific manner, defining BM structure and function. Collagen IV has been shown to bind to platelets, hepatocytes, keratinocytes, endothelial, mesangial, pancreatic cells and some tumor cells.

Tissue injury in the autoimmune disease Goodpasture syndrome is due to pathogenic autoantibodies targeting the Collagen IV α3 chain . Mutations in COL4A5 are associated with Alport syndrome.

Komponenten

All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type IV collagen occurs only in the basement membranes and contains up to six genetically distinct a-chains.

Vorsicht

This product should be stored desiccated at -20°C, and will retain activity in these conditions for 3 years.

Angaben zur Herstellung

This powder can be reconstituted in sterile .5 M acetic acid, PBS or water at 1 mg/mL. A PBS solution will be stable for at least one year at -20°C.

Hinweis zur Analyse

An SDS polyacrylamide gel electrophoresis test run under reducing conditions consistent with basement membrane collagen yields three major bands.

Sonstige Hinweise

Kollagen wird in eine Reihe an strukturell und genetisch verschiedenen Typen unterteilt. Wir nutzen die Nomenklatur, die von Bornstein und Traub vorgeschlagen wurde. Achten Sie darauf, dass Sie die Typenbezeichnungen von Sigma nicht mit anerkannten Typenklassifizierungen von Kollagen verwechseln.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable