AB0300

Alkaline Phosphatase Blue Membrane Substrate Solution

alkaline phosphatase substrate, solution

• UNSPSC-Code: 12352204
• eCl@ss: 42010105
• NACRES: NA.83

Eigenschaften

• product name: Alkaline Phosphatase Blue Membrane Substrate Solution, sufficient for 400 mL working substrate
• Form: solution
• Qualitätsniveau: 200
• Verwendung: sufficient for 400 mL working substrate
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

Alkaline phosphatase, a metalloenzyme is a member of ectonucleotidases family. It is present in bile ducts, bone, intestine, placenta and tumors.

Anwendung

Alkaline Phosphatase Blue Membrane Substrate Solution has been used:

• to analyse direct alkaline phosphatase (AP) activity
• to perform alkaline phosphatase assays with colorimetric system
• to analyse direct alkaline phosphatase (AP) activity of the induced pluripotent stem cells (iPSC) line LND554SV.3

Prior to reaction with alkaline phosphatase, the BCIP^® reagent is colorless to faint blue and the Nitro BT reagent is yellow. The two-component mixture develops a bluish-purple product with alkaline phosphatase in membrane-type assays. This substrate is not recommended for ELISA assays.

Physikalische Form

A 2 component kit sufficient for preparing 400 mL of working substrate.

Supplied as a two-component buffered alkaline phosphatase substrate containing bromochloroindolyl phosphate (BCIP^®) and Nitro Blue Tetrazolium (NBT/Thiazolyl Blue/Nitro BT)

Rechtliche Hinweise

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable