56500

GenElute^™ Agarose-Spinsäulen

sufficient for 70 purifications

• Synonym(e): DNA purification from agarose gel, Gen Elute
• UNSPSC-Code: 41106300
• NACRES: NA.52

Eigenschaften

• Verwendung: sufficient for 70 purifications

Beschreibung

Allgemeine Beschreibung

GenElute Agarose Spin Columns allow for the purification of linear DNA from agarose gels. The typical recovery is 40 to 45% for 100 bp to 10 kb DNA fragments. The recovery decreases as the fragment size increases.

Anwendung

The purified DNA can be used in most downstream molecular biology applications including ligation, PCR, restriction digest, cloning, labeling and hybridization.

Leistungsmerkmale und Vorteile

• One-step, 10 minute protocol
• No melting or digestion of agarose required
• Simpler and quicker than bind and elute or electroelution methods
• No ethanol precipitation
• Typical recovery of 40 to 45% for DNA fragments from 100 bp to 10 kb (Figs. 1 and 2)

Sonstige Hinweise

GenElute^™ Agarose Spin Columns isolate DNA by centrifugation/filtration.

For additional information, please see www.sigma-aldrich.com/post-reaction.

Prinzip

GenElute Agarose Spin Columns are designed for the rapid purification of linear DNA (100 bp to 10 kb) from agarose gels. The DNA band is excised from an agarose gel and loaded into the spin column. Embedded within the base of the column are a series of membranes and filters that hold agarose and impurities back while allowing DNA to selectively pass through the column into a collection tube during a 10 minute centrifugation. GenElute Agarose Spin Columns eliminate the need for silica-based resins, DEAE or toxic organic solvents such as phenol and chloroform. There is no melting, electroelution or enzymatic digestion of the agarose gel.

Rechtliche Hinweise

GenElute is a trademark of Sigma-Aldrich Co. LLC