Die Mega Stokes Farbstoffe, welche von Luminochem lizenziert wurden, ermöglichen eine einfache Kopplung an Biomoleküle basierend auf dem „Click" Prinzip (für Kupfer-Katalysierte und kupferfreie Reaktionen).Sie decken das gesamte sichtbare Emissionsspektrum bis hin zum nahen Infra-Rot Bereich ab. Die Farbstoffe zeigen teilweise einen starken Stokes-shift von bis zu 150 nm auf und ermöglichen so auch spezielle Anwendung, wie z.B. FRET oder Mehrfarben-Detektionen.
Anwendung
Alkyne MegaStokes dye 673 is a reagent used for copper catalyzed click labeling.
Verpackung
Bottomless glass bottle. Contents are inside inserted fused cone.
The alkyne-azide Cu(I)-catalyzed Huisgen cycloaddition, a click-type reaction, was used to label a double-stranded oligonucleotide (siRNA) with fluorine-18. An alkyne solid support CPG for the preparation of monostranded oligonucleotides functionalized with alkyne has been developed. Two complementary azide labeling agents
Cancer biotherapy & radiopharmaceuticals, 24(3), 289-302 (2009-06-23)
This update summarizes the growing application of "click" chemistry in diverse areas such as bioconjugation, drug discovery, materials science, and radiochemistry. This update also discusses click chemistry reactions that proceed rapidly with high selectivity, specificity, and yield. Two important characteristics
Methods (San Diego, Calif.), 48(1), 8-13 (2009-02-28)
We describe a method for synchronization of the cell cycle in the bacterium Escherichia coli. Treatment of asynchronous cultures with the amino acid analog, dl-serine hydroxamate, induces the stringent response, with concomitant arrest of DNA replication at initiation. Following release
Biophysical and mechanistic investigation of RNA function requires site-specific incorporation of spectroscopic and chemical probes, which is difficult to achieve using current technologies. We have in vitro reconstituted a functional box C/D small ribonucleoprotein RNA 2'-O-methyltransferase (C/D RNP) from the
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