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Roche

DIG Oligonucleotide 3′-End Labeling Kit, 2nd generation

greener alternative

sufficient for 25 labeling reactions (100 pmol of oligonucleotides per assay; 1 ug of a 30-mer oligonucleotide), storage condition avoid repeated freeze/thaw cycles

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About This Item

UNSPSC-Code:
41105500
NACRES:
NA.55

Verwendung

sufficient for 25 labeling reactions (100 pmol of oligonucleotides per assay; 1 ug of a 30-mer oligonucleotide)

Qualitätsniveau

100

Hersteller/Markenname

Roche

Grünere Alternativprodukt-Eigenschaften

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Grünere Alternativprodukt-Kategorie

, Aligned

Versandbedingung

dry ice

Allgemeine Beschreibung

The DIG Oligonucleotide 3′-End Labeling Kit, 2nd generation employs the enzyme terminal transferase. It catalyzes the addition of single digoxigenin-labeled dideoxyuridine triphosphates (ddUTP) to the 3′-OH end of oligonucleotides. Thus, helps in labeling oligonucleotides.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Anwendung

DIG Oligonucleotide 3′-End Labeling Kit, 2nd generation is for 3′-end labeling of oligonucleotides from 14 to 100 nucleotides in length with DIG-11-ddUTP and recombinant terminal transferase.
DIG-labeled oligonucleotides has been used in a variety of hybridization techniques:
  • dot/slot blots
  • colony/ plaque hybridizations
  • Southern blots/ northern blots
  • in situ hybridizations

Leistungsmerkmale und Vorteile

  • Fast hybridization kinetics, due to the small size of oligonucleotides
  • Single-stranded probes, no renaturation during hybridization
  • Sequence can be designed according to the experiment
  • Specially suited for in situ hybridization; due to their small size, the oligonucleotides readily diffuse into fixed tissues and cells

Verpackung

1 kit containing 9 components

Qualität

Function tested in a dot blot.

Prinzip

One DIG-ddUTP molecule is added to the 3′-end of oligonucleotides by recombinant Terminal Transferase. This guarantees a very specific and distinct hybridization signal, which is detected by an enzyme-linked immunoassay with anti-DIG-AP antibody conjugate, and a color or chemiluminescence reaction.

Angaben zur Herstellung

Activator: sodium sulfate, Tris
Working concentration: Oligonucleotides: 100 pmol
Up to 100 pmol (1 μg of a 30-mer) oligonucleotide can be labeled in a single standard labeling reaction.
Assay Time: The complete procedure from labeling the oligonucleotide to hybridization and detection of the first visible signal can be accomplished within less than 24 hours.

Sample Materials
Oligonucleotides of a length from 14 to 100 nucleotides, purified by HPLC or gel electrophoresis

Lagerung und Haltbarkeit

Store at -15–-25 °C. (unopened kit)

Sonstige Hinweise

For life science research only. Not for use in diagnostic procedures.

Nur Kit-Komponenten

Produkt-Nr.
Beschreibung
  • Reaction Buffer 5x concentrated
  • CoCl<sub>2</sub> Solution 25 mM
  • DIG-ddUTP Solution 1 mM
  • Recombinant Terminal Transferase 400 U/μl
  • Control Oligonucleotide, unlabeled 20 pmol/μl
  • Oligonucleotide, DIG-ddUTP labeled 2.5 pmol/μl
  • Control DNA, 2.5 pmol/μl pUC 18 DNA, supercoiled
  • Glycogen Solution 20 mg/ml
  • DNA Dilution Buffer, 50 μg/ml fish sperm DNA
Alle anzeigen (9)

Signalwort

Danger

Gefahreneinstufungen

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1B Inhalation - Repr. 1B

Lagerklassenschlüssel

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Flammpunkt (°F)

does not flash

Flammpunkt (°C)

does not flash

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Ming-Xiang Zhang et al.
Proceedings of the National Academy of Sciences of the United States of America, 102(47), 16967-16972 (2005-11-15)
Repeats (27-nt) in intron 4 have been shown to play a cis-acting role in endothelial nitric oxide synthase (eNOS) promoter activity. We hypothesize that the 27-nt repeats could be the source of small nuclear RNA specifically regulating eNOS expression. In
Rosa M Porreca et al.
Nucleic acids research, 46(9), 4533-4545 (2018-03-10)
Telomere maintenance protects the cell against genome instability and senescence. Accelerated telomere attrition is a characteristic of premature aging syndromes including Dyskeratosis congenita (DC). Mutations in hRTEL1 are associated with a severe form of DC called Hoyeraal-Hreidarsson syndrome (HHS). HHS
Fengzhen Liu et al.
Cell reports, 35(10), 109225-109225 (2021-06-10)
Maintaining a suitable level of sensitivity to environmental cues is crucial for proper function of adult stem cells. Here, we explore how the intrinsic sensitivity of skin hair follicle (HF) progenitors to growth stimuli is dynamically regulated. We discover miR-24
Lydgia A Jackson et al.
BMC genomics, 18(1), 317-317 (2017-04-23)
For most pathogens, iron (Fe) homeostasis is crucial for maintenance within the host and the ability to cause disease. The primary transcriptional regulator that controls intracellular Fe levels is the Fur (ferric uptake regulator) protein, which exerts its action on
Pieter J Oort et al.
Molecular and cellular endocrinology, 276(1-2), 24-35 (2007-08-11)
Tumor suppressor candidate 5 (Tusc5, also termed brain endothelial cell derived gene-1 or BEC-1), a CD225 domain-containing, cold-repressed gene identified during brown adipose tissue (BAT) transcriptome analyses was found to be robustly-expressed in mouse white adipose tissue (WAT) and BAT
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